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Green Tea Hyperglycemia
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1. Hyperoxia, hyperglycemia, and photoreceptor sensitivity in normal and diabetic subjects.
Match Strength: 8.508

The aim of this study was to investigate the effects of an increase in the saturation of blood oxygen (SaO2) and/or serum glucose on photoreceptor sensitivity in normal subjects and in patients with diabetes mellitus. We monitored cone and rod sensitivity by recording dark-adaptation curves to both green and red test stimuli while inhaling either air (20% O2 + 80% N2) or 100% oxygen in 12 normal subjects and 12 diabetic patients with no (10) or mild (2) retinopathy. We also repeated the experiment in 10 of the normal subjects under hyperglycemia (mean serum glucose: 161 mg/dl). Results show ... Read More »
» Published in Vis Neurosci. 2006 May-Aug;23(3-4):651-61.

2. Metformin Prevents Glucose-Induced Protein Kinase C-Beta2 Activation in Human Umbilical Vein Endothelial Cells Through an Antioxidant Mechanism
Match Strength: 7.250

Hyperglycemia determines the vascular complications of diabetes through different mechanisms: one of these is excessive activation of the isoform beta2 of protein kinase C (PKC-beta2). Metformin, a widely used antidiabetic agent, is associated with decreased cardiovascular mortality in obese type 2 diabetic patients. Therefore, we assessed the role of metformin in glucose-induced activation of PKC-beta2 and determined the mechanism of its effect in human umbilical venous endothelial cells grown to either normo- (5 mmol/l) or hyperglycemia (10 mmol/l) and moderately and acutely exposed to 25 ... Read More »
» Published in Diabetes. 2005 Apr;54(4):1123-31.

3. Green tea supplementation ameliorates insulin resistance and increases glucose transporter IV content in a fructose-fed rat model.
Match Strength: 7.078

BACKGROUND: Sprague-Dawley rats fed a fructose-rich diet exhibit insulin resistance and hypertension, a pathologic status resembling human type II diabetes mellitus, and are an excellent laboratory animal model for research on insulin action and the development of hypertension. Since green tea has numerous beneficial effects, we tested its effect on fructose-fed rats. AIM: The present study was therefore designed to further evaluate the effects of green tea supplementation on insulin resistance, hypertension, and the glucose transporters I and IV contents in adipose tissue in the fructose-fed ... Read More »
» Published in Eur J Nutr. 2004 Apr;43(2):116-24. Epub 2004 Jan 6.

4. Platelet Hyperactivity And Abnormal Ca(2+) Homeostasis In Diabetes Mellitus
Match Strength: 6.296

We sought to determine the mechanisms for hyperactivity and abnormal platelet Ca(2+) homeostasis in diabetes. The glycosylated Hb (HbA(1c)) level was used as an index of glycemic control. Human platelets were loaded with Ca- green-fura red, and cytosolic Ca(2+) ([Ca(2+)](i)) and aggregation were simultaneously measured. In the first series of experiments, the platelets from diabetic and normal subjects were compared for the ability to release Ca(2+) or to promote Ca(2+) influx. A potent and relatively specific inhibitor of Na(+)/Ca(2+) exchange, 5-(4-chlorobenzyl)-2',4'-dimethylbenzamil (CB ... Read More »
» Published in Am J Physiol Heart Circ Physiol. 2001 Apr;280(4):H1480-9.

5. Effect of high glucose on cellular proliferation and lipid peroxidation in cultured Vero cells.
Match Strength: 6.227

Nephropathy is common among the diabetic population. However, the molecular mechanisms by which hyperglycemia can cause alteration in kidney structure and function is not known. In this study, we examined the effect of high glucose levels on cellular growth and membrane lipid peroxidation in Vero cells, an African green monkey kidney cell line. Cell growth was assessed by a tetrazolium salt reduction test (MTT); and lipid peroxidation was measured by thiobarbituric acid reactivity. Our results show that elevated levels of glucose (25, 50 and 100 mM) can cause up to 50% reduction in cell growth ... Read More »
» Published in Horm Metab Res. 1994 Jul;26(7):322-5.

6. Artemisia herba-alba Asso (Asteraceae) Has Equivalent Effects to Green and Black Tea Decoctions on Antioxidant Processes and Some Metabolic Parameters in Rats.
Match Strength: 6.141

Aims: The objective was to compare the long-term effects of Artemisia herba-alba Asso decoction with a green or black tea decoction, prepared without sugar, on the antioxidant processes in rats. Methods: The direct parameters used in the control of antioxidant processes were total antioxidant status, glutathione peroxidase activity and conjugated dienes, as early markers of lipid peroxidation. However, the indirect parameters used in this control were the body weight gains, plasma glucose and lipid concentrations, iron, copper and zinc status. Results: After 9 weeks, artemisia or tea ... Read More »
» Published in Ann Nutr Metab. 2007 Jun 18;51(3):216-222

7. Protective Effects of Epicatechin Against the Toxic Effects of Streptozotocin on Rat Pancreatic Islets: in Vivo and in Vitro
Match Strength: 5.445

INTRODUCTION: Green tea catechins have diverse pharmacological effects such as anticarcinogenic and antioxidant activities. AIM: To study the protective effects of green tea (-)-epicatechin (EC) against the toxic effects of streptozotocin (STZ), a selective beta cell toxin, on pancreatic islets in vivo and in vitro. METHODOLOGY: Rats were randomly divided into four groups: control, EC (30 mg/kg)-treated, STZ (60 mg/kg)-treated, and EC plus STZ (same doses; EC+STZ)-treated rats. EC was administered twice a day for 6 days, and a single injection of STZ was used. In EC+STZ-treated rats, EC was ... Read More »
» Published in Pancreas. 2003 Apr;26(3):292-9.

8. Sustained exposure to high glucose concentrations modifies glucose signaling and the mechanics of secretory vesicle fusion in primary rat pancreatic beta-cells.
Match Strength: 5.304

The mechanism(s) by which chronic hyperglycemia impairs glucose-stimulated insulin secretion is poorly defined. Here, we compare the "nanomechanics" of single exocytotic events in primary rat pancreatic beta-cells cultured for 48 h at optimal (10 mmol/l) or elevated (30 mmol/l) glucose concentrations. Cargo release was imaged by total internal reflection fluorescence microscopy of lumen-targeted probes (neuropeptide Y [NPY]-pH-insensitive yellow fluorescent protein [NPY-Venus] or NPY-monomeric red fluorescent protein), while the fate of the vesicle membrane was reported simultaneously with ... Read More »
» Published in Diabetes. 2006 Apr;55(4):1057-65.

9. Bone Marrow (BM) Transplantation Promotes {beta} Cell Regeneration after Acute Injury through BM Cell Mobilization.
Match Strength: 4.955

There is controversy regarding the roles of bone marrow (BM)-derived cells in pancreatic beta cell regeneration. To examine these roles in vivo, mice were treated with streptozotocin (STZ), followed by bone marrow transplantation (BMT; lethal irradiation and subsequent BM cell infusion) from green fluorescence protein (GFP) transgenic mice. BMT improved STZ-induced hyperglycemia, nearly normalizing glucose levels, with partially restored pancreatic islet number and size, while simple BM cell infusion without pre-irradiation had no effects. In post-BMT mice, most islets were located near ... Read More »
» Published in Endocrinology. 2007 Jan 25;

10. Effects of (-)-epigallocatechin-3-gallate on pancreatic beta-cell damage in streptozotocin-induced diabetic rats.
Match Strength: 4.621

Oxygen free radicals, which are produced as a result of chronic hyperglycemia, are implicated in many diabetic complications. (-)-Epigallocatechin-3-gallate (EGCG), a green tea polyphenol, has been shown to have both antioxidant and pro-oxidant activities, but its effect on oxidative stress of pancreatic beta cells in vivo is unclear. Here we evaluated the effects of nanomolar concentrations of EGCG on beta-cell survival and the response to high glucose loading in streptozotocin-induced diabetic rats. A 4-day intraperitoneal treatment with EGCG (5 mg/kg/day) further impaired the beta-cell ... Read More »
» Published in Eur J Pharmacol. 2006 Jul 10;541(1-2):115-21. Epub 2006 Jun 12.

11. Therapeutic potential of (-)-epigallocatechin 3-O-gallate on renal damage in diabetic nephropathy model rats.
Match Strength: 4.293

Previous investigations have demonstrated that green tea polyphenols and partially hydrolyzed guar gum as dietary fiber have antioxidative and hypolipidemic activity, respectively, supporting their reduction of risk factors in the course of diabetic nephropathy via a hypoglycemic effect and ameliorating the decline of renal function through their combined administration to rats with subtotal nephrectomy plus streptozotocin (STZ) injection. As a further study, we examined whether (-)-epigallocatechin 3-O-gallate (EGCg), the main polyphenolic compound, could ameliorate the development of ... Read More »
» Published in J Pharmacol Exp Ther. 2006 Oct;319(1):228-36. Epub 2006 Jul 11.

12. Cell biology assessment of glucokinase mutations V62M and G72R in pancreatic beta-cells: evidence for cellular instability of catalytic activity.
Match Strength: 3.788

Mutations in the glucokinase (GK) gene cause defects in blood glucose homeostasis. In some cases (V62M and G72R), the phenotype cannot be explained by altered enzyme kinetics or protein instability. We used transient and stable expression of green fluorescent protein (GFP) GK chimaeras in MIN6 beta-cells to study the phenotype defect of V62M and G72R. GK activity in lysates of MIN6 cell lines stably expressing wild-type or mutant GFP GK showed the expected affinity for glucose and response to pharmacological activators, indicating the expression of catalytically active enzymes. MIN6 cells ... Read More »
» Published in Diabetes. 2007 Jul;56(7):1773-82. Epub 2007 Mar 27.

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