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Green Tea Cholesterol
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1. A green tea extract lowers plasma cholesterol by inhibiting cholesterol synthesis and upregulating the LDL receptor in the cholesterol-fed rabbit.
Match Strength: 12.416

Green tea extracts enriched in catechins decrease plasma cholesterol in hamsters, mice and rats. The aims of this study were to determine whether a catechin-enriched extract of green tea could lower plasma cholesterol in the cholesterol-fed rabbit and to determine the mechanism of action. Four groups of six New Zealand White rabbits were initially made hypercholesterolaemic by feeding a 0.25% (w/w) cholesterol diet for 2 weeks before the diet was supplemented with a catechin extract from green tea at 0, 0.5, 1 or 2% (w/w) for 4 weeks. Administration of the crude catechin extract from green tea ... Read More »
» Published in Atherosclerosis. 2006 Sep 11;

2. Modulation of cholesterol metabolism by the green tea polyphenol (-)-epigallocatechin gallate in cultured human liver (HepG2) cells.
Match Strength: 11.436

Epidemiological and animal studies have found that green tea is associated with lower plasma cholesterol. This study aimed to further elucidate how green tea modulates cholesterol metabolism. When HepG2 cells were incubated with the main green tea constituents, the catechins, epigallocatechin gallate (EGCG) was the only catechin to increase LDL receptor binding activity (3-fold) and protein (2.5-fold) above controls. EGCG increased the conversion of sterol regulatory element binding protein-1 (SREBP-1) to its active form (+56%) and lowered the cellular cholesterol concentration (-28%). At 50 ... Read More »
» Published in J Agric Food Chem. 2006 Mar 8;54(5):1621-6.

3. A green tea catechin extract upregulates the hepatic low-density lipoprotein receptor in rats.
Match Strength: 11.014

Green tea extracts have hypocholesterolaemic properties in epidemiological and animal intervention studies. Upregulation of the low-density lipoprotein (LDL) receptor may be one mechanism to explain this as it is the main way cholesterol is removed from the circulation. This study aimed to determine if a green tea extract could upregulate the hepatic LDL receptor in vivo in the rat. A green tea extract (GTE) enriched in its anti-oxidant constituents, the catechins, was fed to rats (n = 6) at concentrations of either 0, 0.5, 1.0 or 2.0% (w/w) mixed in with their normal chow along with 0.25% (w ... Read More »
» Published in Lipids. 2007 Jul;42(7):621-7. Epub 2007 Jun 21.

4. Green tea upregulates the low-density lipoprotein receptor through the sterol-regulated element binding Protein in HepG2 liver cells.
Match Strength: 10.431

Green tea from Camellia sinensis lowers plasma cholesterol in animal models of hypercholesterolemia. The aim of this study was to determine the effects of green tea on the expression of the hepatic low-density lipoprotein (LDL) receptor, a cell surface protein involved in the control of plasma cholesterol. Incubating human HepG2 liver cells in culture with green tea increased both LDL receptor binding activity and protein. An ethyl acetate extract of green tea, containing 70% (w/w) catechins, also increased the LDL receptor binding activity, protein, and mRNA, indicating that (1) the effect ... Read More »
» Published in J Agric Food Chem. 2001 Nov;49(11):5639-45.

5. Green tea as inhibitor of the intestinal absorption of lipids: potential mechanism for its lipid-lowering effect.
Match Strength: 10.095

Animal and epidemiological studies suggest that green tea catechins may reduce the risk of cardiovascular diseases [e.g., coronary heart disease (CHD)]. The health benefit of green tea has been attributed to its antioxidant and anti-inflammatory properties; however, considerable evidence suggests that green tea and its catechins may reduce the risk of CHD by lowering the plasma levels of cholesterol and triglyceride. Although the mechanism underlying such effect of green tea is yet to be determined, it is evident from in vitro and in vivo studies that green tea or catechins inhibit the ... Read More »
» Published in J Nutr Biochem. 2007 Mar;18(3):179-83.

6. Hypocholesterolemic effects of phenolic-rich extracts of Chemlali olive cultivar in rats fed a cholesterol-rich diet.
Match Strength: 9.480

This study was designed to test the lipid-lowering and the antioxidative activities of green and black olive phenolic extracts. Wistar rats fed a standard laboratory diet or a cholesterol-rich diet for 16 weeks were used. The serum lipid levels, the malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) as well as that of catalase (CAT) were examined. The cholesterol-rich diet induced hypercholesterolemia that was manifested in the elevation of total cholesterol (TC) and low density lipoprotein cholesterol (LDL-C). Administration of aqueous methanol and ethyl acetate extracts of ... Read More »
» Published in Bioorg Med Chem. 2005 Sep 15;13(18):5362-70.

7. No effect of consumption of green and black tea on plasma lipid and antioxidant levels and on LDL oxidation in smokers.
Match Strength: 9.104

Intake of flavonoids is associated with a reduced cardiovascular risk. Oxidation of LDL is a major step in atherogenesis, and antioxidants may protect LDL from oxidation. Because tea is an important source of flavonoids, which are strong antioxidants, we have assessed in a randomized, placebo-controlled study the effect of consumption of black and green tea and of intake of isolated green tea polyphenols on LDL oxidation ex vivo and on plasma levels of antioxidants and lipids. Healthy male and female smokers (aged 34+/-12 years, 13 to 16 per group) consumed during a 4-week period 6 cups (900 ... Read More »
» Published in Arterioscler Thromb Vasc Biol. 1998 May;18(5):833-41.

8. Lipids, lipoproteins, and endocrine profiles during pregnancy in the African green monkey (Cercopithecus aethiops).
Match Strength: 9.013

In an attempt to establish relationships between the endocrine and lipid metabolism during pregnancy, the changes in total plasma cholesterol (TPC) and lipoprotein cholesterol that occur during pregnancy in the African green monkey were investigated longitudinally in ten females in relation to the changes in progesterone, estradiol, and fasting insulin concentrations. Respective means for TPC, high-density lipoprotein (HDL) cholesterol, and low-density lipoprotein (LDL) plus very low-density lipoprotein (VLDL) cholesterol were 343 +/- 35, 108 +/- 9, and 235 +/- 36 mg/dL prior to the estimated ... Read More »
» Published in Metabolism. 1984 Sep;33(9):840-4.

9. Green tea consumption and serum malondialdehyde-modified LDL concentrations in healthy subjects.
Match Strength: 8.689

OBJECTIVE: Green tea was shown to inhibit LDL oxidation, platelet aggregation, and matrix metalloproteinases (MMPs) activities in vitro. We tried to elucidate whether or not green tea consumption may have these effects in vivo, which may be protective against atherosclerotic disease. METHODS: We measured serum malondialdehyde-modified LDL (MDA-LDL) concentrations and urine 8-epi-prostaglandin (PG) F(2alpha) in 22 healthy male nonsmokers. They drank 7 cups/day of water for 2 weeks and drank 7 cups/day of green tea for the next 2 weeks. Regarding platelet aggregation, plasma thromboxane B(2) ... Read More »
» Published in J Am Coll Nutr. 2005 Oct;24(5):342-6.

10. Medicinal Benefits of Green Tea: Part I. Review of Noncancer Health Benefits
Match Strength: 7.855

Tea, in the form of green or black tea, is one of the most widely consumed beverages in the world. Extracts of tea leaves also are sold as dietary supplements. However, with the increasing interest in the health properties of tea and a significant rise in scientific investigation, this review covers recent findings on the medicinal properties and noncancer health benefits of both green and black tea. In Part II, a review of anticancer properties of green tea extracts is presented. Green tea contains a unique set of catechins that possess biological activity in antioxidant, anti-angiogenesis, ... Read More »
» Published in J Altern Complement Med. 2005 Jun;11(3):521-8.

11. Effects of green tea, black tea and dietary lipophilic antioxidants on LDL oxidizability and atherosclerosis in hypercholesterolaemic rabbits.
Match Strength: 7.839

The hypothesis that tea or dietary lipid-soluble antioxidants reduce atherogenesis by lowering the oxidizability of low-density lipoprotein (LDL) was investigated. Five groups of 20 female New Zealand white rabbits were fed a restricted amount of a high-fat (30 en%) semipurified diet supplemented with cholesterol (0.15%, w/w) for 21 weeks. The vitamin E content of the control diet was 40 mg/kg diet. The animals received either green tea or black tea in their drinking water or vitamin E (200 mg/kg diet) or beta-carotene (20 mg/kg). The serum cholesterol concentrations (in the order of 18-23 ... Read More »
» Published in Atherosclerosis. 1997 Nov;135(1):37-47.

12. Green tea extract impedes dyslipidaemia and development of cardiac dysfunction in streptozotocin-diabetic rats.
Match Strength: 7.660

1. The efficacy of green tea extract (GTE) on serum and cardiac lipids was investigated in streptozotocin (STZ)-diabetic rats. 2. Diabetes was induced in rats by a single intraperitoneal injection of STZ (60 mg/kg bodyweight). Six weeks after the induction of diabetes, GTE was administered orally for 4 weeks (300 mg/kg bodyweight daily). Bodyweight, heart weight, heart weight : bodyweight ratio, blood glucose, serum and cardiac lipids were determined in experimental rats. 3. In diabetic rats, there was a significant decrease in bodyweight with an increase in heart weight : bodyweight ratio and ... Read More »
» Published in Clin Exp Pharmacol Physiol. 2006 Dec;33(12):1184-9.

13. CoQ9 potentiates green tea antioxidant activities in Wistar rats.
Match Strength: 7.645

Green tea (Camellia sinensis), and CoQ(9 )when given to Wistar rats produced a partial reversal on reserpine induced oxidative stress and liver damage. Green tea, with its abundant polyphenol (-)Epigallocatechin 3-gallate (ECGC) and other catechins, is known for its antioxidative characteristics influencing lipid metabolism. Ubiquinone, abundant in heart muscle, is also a potent antioxidant with known effects in numerous pathologies. However the combined effect of ECGC and ubiquninone has not been reported. In the present study we found that green tea extract, when given in combination with ... Read More »
» Published in Biofactors. 2005;25(1-4):255-9.

14. Lung cancer risk and red meat consumption among Iowa women.
Match Strength: 7.444

OBJECTIVE: Some epidemiologic studies suggest that diets high in total fat, saturated fat, or cholesterol are associated with increased risk of lung cancer. Others suggest that diets high in red meat consumption, particularly well-done red meat, are a lung cancer risk factor. In Iowa, we had the opportunity to investigate concurrently the role of meat intake and macronutrients in lung cancer etiology. METHODS: A population-based case-control study of both non-smoking and smoking women was conducted in Iowa. A 70-item food frequency questionnaire (FFQ) was completed by 360 cases and 574 ... Read More »
» Published in Lung Cancer. 2001 Oct;34(1):37-46.

15. Comparative studies on the hypolipidemic and growth suppressive effects of oolong, black, pu-erh, and green tea leaves in rats.
Match Strength: 7.379

The four major commercial teas, oolong, black, pu-erh, and green teas, have been manufactured in southeast Asia. In this study, we evaluated the growth suppressive and hypolipidemic effect of these four different tea leaves by oral feeding to male Sprague-Dawley rats for 30 weeks. The results showed that the suppression of body weights of tea leaves-fed groups were in the order: oolong tea > pu-erh tea > black tea > green tea. Pu-erh tea and oolong tea could lower the levels of triglyceride more significantly than that of green tea and black tea, but pu-erh tea and green tea were more ... Read More »
» Published in J Agric Food Chem. 2005 Jan 26;53(2):480-9.

16. Effect of green tea on angiogenesis and severity of atherosclerosis in cholesterol-fed rabbit.
Match Strength: 7.332

BACKGROUND AND AIMS: Since the development of the atherosclerotic plaque requires the growth of new microvessels in the plaque itself (vasa vasorum), we postulated that green tea may exert an anti-atherogenic effect. METHODS AND RESULTS: Thirteen male New Zealand white rabbits were studied for 17 weeks. All rabbits were fed an hypecholesterolemic diet. After 2 weeks of adaptation rabbits were randomly assigned into two groups. Animals in Group A were fed the hypercholesterolemic diet and received plain tap water ad libitum. Animals in Group B were fed with the same diet and furthermore ... Read More »
» Published in Vascul Pharmacol. 2006 Jun;44(6):461-3. Epub 2006 May 11.

17. Habitual coffee but not green tea consumption is inversely associated with metabolic syndrome An epidemiological study in a general Japanese population.
Match Strength: 7.309

In Japan, metabolic syndrome used to be rare, and the level of coffee consumption was low. However, the Japanese life style has been changing rapidly, and these changes have been associated with a steady increase in the frequency of metabolic syndrome and with greater consumption of coffee. We examined the relationship between metabolic syndrome and the consumption of coffee or green tea. A total of 1902 Japanese aged over 40 years (785 men and 1117 women) received population-based health check-up in 1999. We measured components of metabolic syndrome (blood pressure, waist circumference, ... Read More »
» Published in Diabetes Res Clin Pract. 2006 Oct 27;

18. A diet rich in green and yellow vegetables inhibits atherosclerosis in mice.
Match Strength: 7.284

Although dietary patterns characterized by a high intake of fruits and vegetables are associated with reduced risk of coronary heart disease, the mechanisms involved are uncertain. We determined the effects of a diet rich in green and yellow vegetables on the development of atherosclerosis, the underlying cause of coronary heart disease, in a mouse model of atherosclerosis, the LDL receptor -/-, apolipoprotein B transgenic mouse. The mice were randomized into 2 diet groups: 1) a vegetable-free control diet (n = 53) and 2) the same diet with 30% (w:w) replaced by an equal-parts mixture of ... Read More »
» Published in J Nutr. 2006 Jul;136(7):1886-9.

19. Cholesterol-linked fluorescent molecular beacons with enhanced cell permeability.
Match Strength: 7.061

We appended pyrene units covalently onto adenosine (forming A(P) units) and then incorporated them into oligonucleotides such that they were positioned in complementary locations in opposite strands in the middle positions of hairpin stems. System 1 (A(P)A(P)) behaves as an effective molecular beacon (MB) that changes color from green to blue upon duplex formation. In addition, we attached a cholesterol unit to a free terminus of one of these hairpins; this approach enhanced the cellular delivery of the modified MB relative to those encountered when using conventional transfection methods. ... Read More »
» Published in Bioconjug Chem. 2006 Sep-Oct;17(5):1151-5.

20. Perfringolysin O, a cholesterol-binding cytolysin, as a probe for lipid rafts.
Match Strength: 7.003

Gaining an understanding of the structural and functional roles of cholesterol in membrane lipid rafts is a critical issue in studies on cellular signaling and because of the possible involvement of lipid rafts in various diseases. We have focused on the potential of perfringolysin O (theta-toxin), a cholesterol-binding cytolysin produced by Clostridium perfringens, as a probe for studies on membrane cholesterol. We prepared a protease-nicked and biotinylated derivative of perfringolysin O (BCtheta) that binds selectively to cholesterol in cholesterol-rich microdomains of cell membranes ... Read More »
» Published in Anaerobe. 2004 Apr;10(2):125-34.

21. Artemisia herba-alba Asso (Asteraceae) Has Equivalent Effects to Green and Black Tea Decoctions on Antioxidant Processes and Some Metabolic Parameters in Rats.
Match Strength: 6.607

Aims: The objective was to compare the long-term effects of Artemisia herba-alba Asso decoction with a green or black tea decoction, prepared without sugar, on the antioxidant processes in rats. Methods: The direct parameters used in the control of antioxidant processes were total antioxidant status, glutathione peroxidase activity and conjugated dienes, as early markers of lipid peroxidation. However, the indirect parameters used in this control were the body weight gains, plasma glucose and lipid concentrations, iron, copper and zinc status. Results: After 9 weeks, artemisia or tea ... Read More »
» Published in Ann Nutr Metab. 2007 Jun 18;51(3):216-222

22. Metabolic Effects of Green Tea and of Phases of Weight Loss
Match Strength: 6.593

The effect of ingestion of green tea (GT) extract along with a low-energy diet (LED) on health-related blood parameters, and the relationships among changes in metabolic parameters and phases of weight loss were assessed. A double-blind, placebo-controlled, parallel design was used. 46 female subjects (BMI 27.7+/-1.8 kg/m(2)) were fed in energy balance from days 1 to 3, followed by a LED with GT (n=23) or placebo (PLAC, n=23) from days 4 to 87. The LED-period consisted of a phase 1 of 4 weeks (days 4-32) followed by a phase 2 of 8 weeks (days 32-87). Body composition and fasting blood samples ... Read More »
» Published in Physiol Behav. 2006 Jan 30;87(1):185-91. Epub 2005 Nov 7.

23. Dietary consumption of green tea catechins attenuate hyperlipidaemia-induced atherosclerosis and systemic organ damage in mice.
Match Strength: 6.563

OBJECTIVE: The effects of consuming green tea catechins on the development of hyperlipidaemia-induced systemic organ damage have not been well studied; we investigated the effect using low density lipoprotein receptor knockout mice. METHODS AND RESULTS: Mice were treated with high cholesterol food containing 0.2 or 4% catechins and they were supplemented for 35 weeks. High plasma cholesterol levels, liver and renal dysfunctions were observed in no catechin fed mice, while chow containing catechin suppressed these levels and damages. Severe atherosclerosis of the aorta, fatty liver and renal ... Read More »
» Published in Acta Cardiol. 2005 Jun;60(3):271-6.

24. Antioxidant polyphenols in almond and its coproducts.
Match Strength: 6.538

Antioxidant efficacy of defatted almond whole seed, brown skin, and green shell cover extracts was evaluated by monitoring inhibition of human low-density lipoprotein (LDL) oxidation, inhibition of DNA scission, and metal ion chelation activities. The total phenolic contents of ethanolic extracts of brown skin and green shell cover of almond were 10 and 9 times higher than that of the whole seed, respectively. Brown skin extract at 50 ppm effectively inhibited copper-induced oxidation of human LDL cholesterol compared to whole seed and green shell cover extracts, which reached the same level ... Read More »
» Published in J Agric Food Chem. 2006 Jan 25;54(2):312-8.

25. Effects of tea polyphenols on emulsification of olive oil in a small intestine model system.
Match Strength: 6.363

Tea catechins have been shown to reduce plasma cholesterol and suppress hypertriacylglycerolemia by reducing triglyceride absorption. However, the mechanism is not yet clear. One of the possible mechanisms is that tea polyphenols may modify dietary fat emulsification in the gastrointestinal tract. The digestive enzyme (lipase) acts on specific emulsion interface properties (droplet size and surface area). Therefore, changes in these properties may modify emulsification and lead to changes in dietary fat digestion and absorption. In this study, the effect of both green and black tea on the ... Read More »
» Published in J Agric Food Chem. 2006 Mar 8;54(5):1906-13.

26. A green tea extract high in catechins reduces body fat and cardiovascular risks in humans.
Match Strength: 6.346

OBJECTIVE: The body fat reducing effect and reduction of risks for cardiovascular disease by a green tea extract (GTE) high in catechins was investigated in humans with typical lifestyles. RESEARCH METHODS AND PROCEDURES: Japanese women and men with visceral fat-type obesity were recruited for the trial. After a 2-week diet run-in period, a 12-week double-blind parallel multicenter trial was performed, in which the subjects ingested green tea containing 583 mg of catechins (catechin group) or 96 mg of catechins (control group) per day. Randomization was stratified by gender and body mass index ... Read More »
» Published in Obesity (Silver Spring). 2007 Jun;15(6):1473-83.

27. Favorable effects of tea on reducing the cognitive deficits and brain morphological changes in senescence-accelerated mice.
Match Strength: 6.328

The present study was carried out to explore the effects of oolong and green teas on improving the memory deficits and brain pathological changes in senescence accelerated-prone mice P8 (SAMP8). Six-month-old mice were supplied with oolong tea, green tea or water as the sole drinking fluid for 16 wk. The memory ability of mice was evaluated by passive and active avoidance tests, while the extent of the brain degeneration was measured by the spongiosis grades and the lipofuscin percentage in the hippocampus. The total grading score and serum biochemical levels were also measured. The results ... Read More »
» Published in J Nutr Sci Vitaminol (Tokyo). 2006 Aug;52(4):266-73.

28. Basal laminar deposit formation in APO B100 transgenic mice: complex interactions between dietary fat, blue light, and vitamin E.
Match Strength: 6.000

PURPOSE: Dietary fat intake has been proposed as a mechanism of sub-RPE deposit formation. It has been demonstrated recently that sub-RPE deposits develop in 16- to 18-month-old C57BL/6 mice fed a high-fat diet and exposed to blue-green light. Hyperlipidemia also develops in these mice after they consume a high-fat diet. Because hyperlipidemia also develops in young C57BL/6 mice that overexpress APO B100, the major apolipoprotein in LDL cholesterol, this research was conducted to determine whether high-fat diet and plasma hyperlipidemia correlate with formation of basal laminar deposits (BLD) ... Read More »
» Published in Invest Ophthalmol Vis Sci. 2004 Jan;45(1):260-6.

29. Green tea modulation of the biochemical and electric properties of rat liver cells that were affected by ethanol and aging.
Match Strength: 5.937

The oxidative stress induced by chronic ethanol consumption, particularly in concert with the aging process, has been implicated in changes in the structure and functions of liver cell components including membrane phospholipids. To counteract such changes, particularly those resulting from lipid peroxidation, antioxidants may be applied. Green tea contains large amounts of polyphenols, mainly catechins, which possess antioxidant properties. The aim of this study was to estimate the efficacy of green tea's influence on the physicochemical and biochemical properties of the rat liver as affected ... Read More »
» Published in Cell Mol Biol Lett. 2004;9(4A):709-21.

30. Green tea modulation of the biochemical and electric properties of rat liver cells that were affected by ethanol and aging.
Match Strength: 5.846

The oxidative stress induced by chronic ethanol consumption, particularly in concert with the aging process, has been implicated in changes in the structure and functions of liver cell components including membrane phospholipids. To counteract such changes, particularly those resulting from lipid peroxidation, antioxidants may be applied. Green tea contains large amounts of polyphenols, mainly catechins, which possess antioxidant properties. The aim of this study was to estimate the efficacy of green tea's influence on the physicochemical and biochemical properties of the rat liver as affected ... Read More »
» Published in Cell Mol Biol Lett. 2004;9(4A):709-21.

31. Effects of Western, Vegetarian, and Japanese Dietary Fat Model Diets with Or Without Green Tea Extract on the Plasma Lipids and Glucose, and Liver Lipids in Mice. a Long-Term Feeding Experiment
Match Strength: 5.844

BACKGROUND/AIMS: The purpose of this study was to investigate the long-term effects of three model diets containing different fats, with or without a small amount of green tea extract (GTE), on plasma lipids and glucose, and liver lipids in mice. METHODS: Male mice (2 months old) fed 10% fat diets with Western (W), Vegetarian (V), and Japanese (J) fat compositions with or without 0.03% GTE for 7 months. RESULTS: The concentrations of plasma and liver total cholesterol in animals fed the W diet were not significantly different from those fed the J diet. Plasma triacylglycerol (TG) ... Read More »
» Published in Ann Nutr Metab. 2004;48(2):95-102. Epub 2004 Feb 25.

32. Cholesterol-sensitive Cdc42 activation regulates actin polymerization for endocytosis via the GEEC pathway.
Match Strength: 5.839

Glycosyl-phosphatidylinositol (GPI)-anchored proteins (GPI-APs) are present at the surface of living cells in cholesterol dependent nanoscale clusters. These clusters appear to act as sorting signals for the selective endocytosis of GPI-APs via a Cdc42-regulated, dynamin and clathrin-independent pinocytic pathway called the GPI-AP-enriched early endosomal compartments (GEECs) pathway. Here we show that endocytosis via the GEECs pathway is inhibited by mild depletion of cholesterol, perturbation of actin polymerization or overexpression of the Cdc42/Rac-interactive-binding (CRIB) motif of ... Read More »
» Published in Traffic. 2007 Jun;8(6):702-17. Epub 2007 Apr 25.

33. A Dominant Negative Form of the Transcription Factor c-Jun Affects Genes That Have Opposing Effects on Lipid Homeostasis in Mice.
Match Strength: 5.784

c-Jun is a transcription factor activated by phosphorylation by the stress-activated protein kinase/c-Jun N-terminal kinase pathway in response to extracellular signals and cytokines. We show that adenovirus-mediated gene transfer of the dominant negative form of c-Jun (dn-c-Jun) in C57BL/6 mice increased greatly apoE hepatic mRNA and plasma levels, increased plasma cholesterol, triglyceride, and very low density lipoprotein levels, and resulted in the accumulation of discoidal high density lipoprotein particles. A similar but more severe phenotype was generated by overexpression of the mouse ... Read More »
» Published in J Biol Chem. 2007 Jul 6;282(27):19556-64. Epub 2007 Apr 24.

34. Mobility of human immunodeficiency virus type 1 Pr55Gag in living cells.
Match Strength: 5.617

Human immunodeficiency virus type 1 (HIV-1) assembly requires the converging of thousands of structural proteins on cellular membranes to form a tightly packed immature virion. The Gag polyprotein contains all of the determinants important for viral assembly and must move around in the cell in order to form particles. This work has focused on Gag mobility in order to provide more insights into the dynamics of particle assembly. Key to these studies was the use of several fluorescently labeled Gag derivatives. We used fluorescence recovery after photobleaching as well as photoactivation to ... Read More »
» Published in J Virol. 2006 Sep;80(17):8796-806.

35. Synthesis of functional protein in liposome.
Match Strength: 5.592

The liposome consisting of eggPC, cholesterol, and DSPE-PEG5000 with a molar ratio of 1.5:1:0.08 was used to entrap cell-free protein synthesis reaction mixture. The synthesis of a mutant green fluorescent protein in the liposome was confirmed by the fluorescence emitted from the liposome on flow cytometry analysis and fluorescence microscopy. The protein synthesized in the liposome is hence functional ... Read More »
» Published in J Biosci Bioeng. 2001;92(6):590-3.

36. Characterization and application of a new optical probe for membrane lipid domains.
Match Strength: 5.527

In this article, we characterize the fluorescence of an environmentally sensitive probe for lipid membranes, di-4-ANEPPDHQ. In large unilamellar lipid vesicles (LUVs), its emission spectrum shifts up to 30 nm to the blue with increasing cholesterol concentration. Independently, it displays a comparable blue shift in liquid-ordered relative to liquid-disordered phases. The cumulative effect is a 60-nm difference in emission spectra for cholesterol containing LUVs in the liquid-ordered state versus cholesterol-free LUVs in the liquid-disordered phase. Given these optical properties, we use di-4 ... Read More »
» Published in Biophys J. 2006 Apr 1;90(7):2563-75. Epub 2006 Jan 13.

37. Mechanisms of hypolipidemic and anti-obesity effects of tea and tea polyphenols.
Match Strength: 5.479

Among the health-promoting effects of tea and tea polyphenols, the cancer-chemopreventive effects in various animal model systems have been intensively investigated; meanwhile, the hypolipidemic and antiobesity effects in animals and humans have also become a hot issue for molecular nutrition and food research. It has been demonstrated that the body weights of rats and their plasma triglyceride, cholesterol, and LDL-cholesterol have been significantly reduced by feedings of oolong, black, pu-erh, and green tea leaves to the animals. It has been suggested that the inhibition of growth and ... Read More »
» Published in Mol Nutr Food Res. 2006 Feb;50(2):211-7.

38. Depletion of membrane cholesterol eliminates the Ca2+-activated component of outward potassium current and decreases membrane capacitance in rat uterine myocytes.
Match Strength: 5.448

Changes in membrane cholesterol content have potent effects on cell signalling and contractility in rat myometrium and other smooth muscles. We have previously shown that depletion of cholesterol with methyl-beta-cyclodextrin (MCD) disrupts caveolar microdomains. The aim of this work was to determine the mechanism underlying the increase in Ca(2+) signalling and contractility occurring in the myometrium with MCD. Patch clamp data obtained on freshly isolated myocytes from the uterus of day 19-21 rats showed that outward K(+) current was significantly reduced by MCD. Membrane capacitance was ... Read More »
» Published in J Physiol. 2007 Jun 1;581(Pt 2):445-56. Epub 2007 Mar 1.

39. Optimal diet for reducing the risk of arteriosclerosis.
Match Strength: 5.399

The primary objectives of current dietary advice for those at risk from coronary artery disease (CAD) focus on progressive restriction of dietary saturated (and trans) fatty acids and cholesterol intake, combined with exercise and achievement of ideal body weight. These principles are endorsed by the official bodies of most western nations concerned with reducing CAD mortality and have recently been reaffirmed by the Adult Treatment Panel of the National Cholesterol Education Program. There has been concern, however, in view of the increasing use of drug therapy, that additional strategies ... Read More »
» Published in Can J Cardiol. 1995 Oct;11 Suppl G:118G-122G.

40. Relationship between smoking habits and serum oxygen radical absorbance capacity and dietary intake in Japanese adults.
Match Strength: 5.225

SUMMARY 1. The present study was undertaken to investigate the effect of smoking habits on dietary intake, serum oxygen radical absorbance capacity (ORAC) and lifestyle in Japanese adults. 2. Lifestyle factors were recorded by questionnaire and dietary intake was measured by a single 24 h food recall. Blood was collected under fasting conditions for analysis. 3. Smokers had lower intakes of vitamin C, fruits and dark green and yellow vegetables (DGYV), higher consumption of alcohol, lower physical activity and lower ORAC values than non-smokers. Body mass index (BMI), serum total cholesterol ... Read More »
» Published in Clin Exp Pharmacol Physiol. 2004 Dec;31 Suppl 2:S35-6.

41. Social context influences androgenic effects on calling in the green treefrog (Hyla cinerea).
Match Strength: 4.987

Courtship behavior in frogs is an ideal model for investigating the relationships among social experience, gonadal steroids, and behavior. Reception of mating calls causes an increase in androgen levels in listening males, and calling, in turn, depends on the presence of androgens. However, previous studies found that androgen replacement does not always restore calling to intact levels, and the relationship between androgens and calling may be context dependent. We examined the influence of androgens on calling behavior in the presence and the absence of social signals in male green treefrogs ... Read More »
» Published in Horm Behav. 2001 Dec;40(4):550-8.

42. Adenovirus-mediated expression of caveolin-1 in mouse liver increases plasma high-density lipoprotein levels.
Match Strength: 4.966

Caveolae are 50-100 nm plasma membrane invaginations, which function in cell signaling and transcytosis, as well as in regulating cellular cholesterol homeostasis. These subcompartments of the plasma membrane are characterized by the presence of caveolin proteins. Recent studies have indicated that caveolae may be involved in the regulation of cellular cholesterol efflux to HDL, as well as selective uptake mediated by SR-BI. In the present study, we have determined the effect of caveolin-1 overexpression in mouse liver on plasma lipoprotein metabolism. We evaluated this effect using an ... Read More »
» Published in Biochemistry. 2001 Sep 11;40(36):10892-900.

43. Prevention of type 2 diabetes in a primary health care setting Interim results from the Greater Green Triangle (GGT) Diabetes Prevention Project.
Match Strength: 4.855

Although clinical trials have shown that lifestyle modifications reduce the risk of type 2 diabetes, translating lessons from trials to primary care remains a challenge. The aim of the study was to evaluate efficacy and feasibility of primary care-based diabetes prevention model with modest resource requirements in rural Australia. Three hundred and eleven subjects with at least a moderate risk of type 2 diabetes participated in a combined dietary and physical activity intervention. Clinical measurements and fasting blood samples were taken at the baseline and after intervention. After 3 ... Read More »
» Published in Diabetes Res Clin Pract. 2006 Oct 25;

44. Hypocholesterolemic effect of soymilk supplementation with usual diet in premenopausal normolipidemic Japanese women.
Match Strength: 4.757

BACKGROUND: The hypocholesterolemic effect of soy protein concentrates on normolipidemic subjects still remains unclear. Our objective is to assess the effect of soymilk supplementation, a whole soy product, with usual diet on serum lipids in normolipidemic subjects. METHODS: We conducted a randomized controlled trial on 60 premenopausal normolipidemic Japanese women. After excluding 8 subjects whose initial serum concentration of total cholesterol or triacylglycerol was higher than 220 mg/dL (5.69 mmol/L) or 160 mg/dL (1.81 mmol/L), respectively, we encouraged the subjects in the soymilk ... Read More »
» Published in Prev Med. 2000 Oct;31(4):308-14.

45. Phosphatidylinositol-3-kinase regulates scavenger receptor class B type I subcellular localization and selective lipid uptake in hepatocytes.
Match Strength: 4.728

OBJECTIVE: The high-density lipoprotein (HDL) receptor scavenger receptor Class B type I (SR-BI) plays a key role in mediating the final step of reverse cholesterol transport. This study examined the possible regulation of hepatic SR-BI by phosphatidylinositol-3-kinase (PI3K), a well known regulator of endocytosis and membrane protein trafficking. METHODS AND RESULTS: SR-BI-dependent HDL selective cholesterol ester uptake in human HepG2 hepatoma cells was decreased (approximately 50%) by the PI3K inhibitors wortmannin and LY294002. Insulin increased selective uptake (approximately 30%), and ... Read More »
» Published in Arterioscler Thromb Vasc Biol. 2006 Sep;26(9):2125-31. Epub 2006 Jun 22.

46. CeVPS-27 is an endosomal protein required for the molting and the endocytic trafficking of the low-density lipoprotein receptor-related protein 1 in Caenorhabditis elegans.
Match Strength: 4.609

Class E vacuolar protein-sorting (Vps) proteins were first described in yeast as being involved in receptor-mediated endocytosis and multivesicular body formation. Inactivation by RNA interference of the class E VPS genes of the nematode Caenorhabditis elegans revealed heterogeneous phenotypes. We have further characterized the role of the essential gene Cevps-27, ortholog of human hepatocyte growth factor-regulated tyrosine kinase substrate, during the development of C. elegans. Use of green fluorescent protein fusion constructs and antibody staining revealed that Cevps-27 localizes to ... Read More »
» Published in Traffic. 2005 Aug;6(8):695-705.

47. Potassium excretion in healthy Japanese women was increased by a dietary intervention utilizing home-parcel delivery of Okinawan vegetables.
Match Strength: 4.556

Potassium, which is abundant in vegetables, is inversely related to blood pressure. Although the situation has changed somewhat in recent years, the Okinawan diet has generally included a large amount of vegetables, and until recently Okinawans had the lowest rates of mortality due to stroke and coronary heart disease in Japan. Based on the hypothesis that these low mortality rates are partly attributable to increased potassium intake resulting from the high vegetable consumption, this study examined whether increasing the consumption of typical yellow-green Okinawan vegetables increases ... Read More »
» Published in Hypertens Res. 2006 Jun;29(6):389-96.

48. Epigallocatechin gallate and caffeine differentially inhibit the intestinal absorption of cholesterol and fat in ovariectomized rats.
Match Strength: 4.489

We conducted this study to determine whether green tea constituents, (-)-epigallocatechin gallate (EGCG) and caffeine, affect the intestinal absorption of cholesterol (CH), fat, and other fat-soluble compounds. Ovariectomized rats with lymph cannula were infused intraduodenally with a lipid emulsion containing 14C-labeled CH (14C-CH), alpha-tocopherol (alpha TOH), triolein, and sodium taurocholate, without (control) or with EGCG, caffeine, or EGCG plus caffeine, in PBS, pH 6.5. The lymphatic total 14C-CH was significantly lowered by EGCG (21.1 +/- 2.1% dose), caffeine (27.9 +/- 1.7% dose), and ... Read More »
» Published in J Nutr. 2006 Nov;136(11):2791-6.

49. Lateral diffusion of TGF-beta type I receptor studied by single-molecule imaging.
Match Strength: 4.475

In this report, we investigated the lateral diffusion of transforming growth factor beta (TGF-beta) type I receptor (TbetaRI) in living cells by imaging and tracking individual green fluorescent protein tagged TbetaRI on the cell membrane. We found that when co-expressed with TGF-beta type II receptor (TbetaRII), the mobility of TbetaRI decreased significantly after TGF-beta1 stimulation. However, in the cells that had been depleted of cholesterol with Nystatin or methyl-beta-cyclodextrin, the diffusion rate of TbetaRI was not changed by TGF-beta1 treatment. Our observations suggest that ... Read More »
» Published in Biochem Biophys Res Commun. 2007 Apr 27;356(1):67-71. Epub 2007 Feb 27.

50. Interrelationships of alpha-tocopherol with plasma lipoproteins in African green monkeys: effects of dietary fats.
Match Strength: 4.469

The distributions of plasma lipoprotein alpha-tocopherol and lipids were studied in African green monkeys consuming diets enriched in saturated, monounsaturated, or polyunsaturated fatty acids. Plasma total alpha-tocopherol concentrations were not different among the animals fed the three diets, whereas plasma total cholesterol concentrations were significantly different among the diet groups. The alpha-tocopherol: total lipid molar ratio in plasma high density lipoproteins (HDL) was significantly higher compared to low density lipoproteins (LDL) and very low plus intermediate-sized low ... Read More »
» Published in J Lipid Res. 1993 Nov;34(11):1863-71.

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