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1. Isolation of Human Corneal Endothelial Cell Precursors and Construction of Cell Sheets by Precursors.
Match Strength: 3.375

PURPOSE:: To review recently published experimental data on human corneal endothelium (CE) precursors. METHODS:: A sphere-forming assay was used for the isolation of human CE precursors from human donor corneas and cultured human CE. CE morphology derived from precursors was compared with that from cultured human CE. RESULTS:: Human CE from donor corneas formed primary and secondary sphere colonies and expressed neural and mesenchymal proteins. The progeny of these colonies had a human-CE-like hexagonal shape and showed transport activity, suggesting that the isolated spheres were indeed ... Read More »
» Published in Cornea. 2006 Dec;25 Suppl 1:S90-S92.

2. JC virus evolution and its association with human populations.
Match Strength: 2.893

The ubiquitous human polyomavirus JC (JCV) is a small double-stranded DNA virus that establishes a persistent infection, and it is often transmitted from parents to children. There are at least 14 subtypes of the virus associated with different human populations. Because of its presumed codivergence with humans, JCV has been used as a genetic marker for human evolution and migration. Codivergence has also been used as a basis for estimating the rate of nucleotide substitution in JCV. We tested the hypothesis of host-virus codivergence by (i) performing a reconciliation analysis of phylogenetic ... Read More »
» Published in J Virol. 2006 Oct;80(20):9928-33.

3. Detection of human-derived fecal pollution in environmental waters by use of a PCR-based human polyomavirus assay.
Match Strength: 2.798

Regulatory agencies mandate the use of fecal coliforms, Escherichia coli or Enterococcus spp., as microbial indicators of recreational water quality. These indicators of fecal pollution do not identify the specific sources of pollution and at times underestimate health risks associated with recreational water use. This study proposes the use of human polyomaviruses (HPyVs), which are widespread among human populations, as indicators of human fecal pollution. A method was developed to concentrate and extract HPyV DNA from environmental water samples and then to amplify it by nested PCR. HPyVs ... Read More »
» Published in Appl Environ Microbiol. 2006 Dec;72(12):7567-74. Epub 2006 Sep 22.

4. Expression, purification, and characterization of stable, recombinant human adenylosuccinate lyase.
Match Strength: 2.726

The full length human adenylosuccinate lyase gene was generated by a PCR method using a plasmid encoding a truncated human enzyme as template, and was cloned into a pET-14b vector. Human adenylosuccinate lyase was overexpressed in Escherichia coli Rosetta 2(DE3)pLysS as an N-terminal histidine-tagged protein and was purified to homogeneity by a nickel-nitriloacetic acid column at room temperature. The histidine tag was removed from the human enzyme by thrombin digestion and the adenylosuccinate lyase was purified by Sephadex G-100 gel filtration. The histidine-tagged and non-tagged ... Read More »
» Published in Protein Expr Purif. 2007 Feb;51(2):227-34. Epub 2006 Aug 9.

5. Review of the economic and quality-of-life burden of cervical human papillomavirus disease.
Match Strength: 2.684

OBJECTIVE: The purpose of this study was to conduct a systematic literature review on the economic burden and health-related quality-of-life impact of cervical human papillomavirus disease. STUDY DESIGN: A systematic review of cost-of-illness studies and health-related quality-of-life studies was conducted. PubMed, Embase, and PsycINFO databases were searched with the use of predefined terms. RESULTS: Nine economic and 24 quality-of-life studies were identified. The annual health care costs of human papillomavirus-related conditions in the United States range from $2.25 to $4.6 billion (2005 ... Read More »
» Published in Am J Obstet Gynecol. 2006 Sep 21;

6. On joy-sorrow: a paradoxical pattern of human becoming.
Match Strength: 2.672

The idea of paradox is central to Parse's human becoming theory. Joy-sorrow is an example of a paradoxical pattern of human becoming which was earlier investigated by Parse, using her research method. Here, the concept is revisited and further explored in light of selected literature, including a classic work on grief and other writings on aesthetic experience. Connections are also drawn between joy-sorrow and the Japanese concept of wabi-sabi. Publication Types: ... Read More »
» Published in Nurs Sci Q. 2006 Oct;19(4):290-1. Comment on: Nurs Sci Q. 2006 Oct;19(4):291-5.

7. Sulfation of the isoflavones genistein and daidzein in human and rat liver and gastrointestinal tract.
Match Strength: 2.626

Phytoestrogens, in particular the isoflavone aglycones genistein and daidzein, are thought to be the bioactive components of soy. Like estrogens, isoflavones can be sulfur-conjugated. However, although isoflavones in the serum are found largely in the form of glucuronide and sulfur conjugates following soy consumption, little is known regarding the relative contributions of sulfotransferases in the liver and small intestine to isoflavone sulfation. Since the sulfates may be deconjugated in target tissues, circulating isoflavone sulfates may act as a source of tissue aglycones. In the current ... Read More »
» Published in J Med Food. 2006 Fall;9(3):348-55.

8. Human telomere biology: pitfalls of moving from the laboratory to epidemiology.
Match Strength: 2.544

Remarkable progress has been made during the last 2 decades in understanding telomere biology at the molecular and cellular levels. Clinical epidemiology research of human telomeres, in contrast, is a discipline just coming into its own. The most important observation in studying human telomere biology is that telomere length is highly variable among humans. Here we explain some of the reasons for this variability and propose several principles that should be considered in conducting epidemiological telomere research. Ignoring these principles could lead to misleading conclusions ... Read More »
» Published in Int J Epidemiol. 2006 Dec;35(6):1424-9. Epub 2006 Sep 22.

9. Humanized mouse models for organ-specific autoimmune diseases.
Match Strength: 2.535

Murine models for human autoimmune diseases are an essential tool for studying pathogenesis and for identifying new therapeutic targets. Mice are not the natural disease host, and conventional models have proved to be poor predictors of efficacy and safety in recent trials aiming to translate drug and biologic treatments to humans. Evidently, further steps towards recapitulating human diseases are urgently needed, for example using transgenic predisposing human HLA allele(s) plus T-cell receptor(s) implicated in a representative patient's autoimmune disease. The latest development - humanizing ... Read More »
» Published in Curr Opin Immunol. 2006 Dec;18(6):704-9. Epub 2006 Sep 27.

10. A genomewide search for ribozymes reveals an HDV-like sequence in the human CPEB3 gene.
Match Strength: 2.528

Ribozymes are thought to have played a pivotal role in the early evolution of life, but relatively few have been identified in modern organisms. We performed an in vitro selection aimed at isolating self-cleaving RNAs from the human genome. The selection yielded several ribozymes, one of which is a conserved mammalian sequence that resides in an intron of the CPEB3 gene, which belongs to a family of genes regulating messenger RNA polyadenylation. The CPEB3 ribozyme is structurally and biochemically related to the human hepatitis delta virus (HDV) ribozymes. The occurrence of this ribozyme ... Read More »
» Published in Science. 2006 Sep 22;313(5794):1788-92. Comment in: Science. 2006 Sep 22;313(5794):1745-7.

11. Towards the development of a pragmatic technique for isolating and differentiating nestin-positive cells from human scalp skin into neuronal and glial cell populations: generating neurons from human skin?
Match Strength: 2.499

Nestin+ hair follicle-associated cells of murine skin can be isolated and differentiated in vitro into neuronal and glial cells. Therefore, we have asked whether human skin also contains nestin+ cells, and whether these can be differentiated in vitro into neuronal and/or glial cell populations. In this methodological pilot study, we show that both are indeed the case - employing purposely only very simple techniques for isolating, propagating, and differentiating nestin+ cells from normal human scalp skin and its appendages that do not require selective microdissection and tissue compartment ... Read More »
» Published in Exp Dermatol. 2006 Oct;15(10):794-800.

12. Kinetic properties and substrate specificities of two recombinant human N-acetylglucosaminyltransferase-IV isozymes.
Match Strength: 2.464

N-acetylglucosaminyltransferase (GnT)-IV catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-glycans. Two human GnT-IV isozymes (GnT-IVa and GnT-IVb) had been identified, which exhibit different expression profiles among human tissues and cancer cell lines. To clarify the enzymatic properties of the respective enzymes, their kinetic parameters were determined using recombinant full-length enzymes expressed in COS7 cells. The K (m) of human GnT-IVb for UDP-GlcNAc was estimated to be 0.24 mM, which is 2-fold higher than that of human ... Read More »
» Published in Glycoconj J. 2006 Nov;23(7-8):473-80.

13. Oceans and human health: Emerging public health risks in the marine environment.
Match Strength: 2.458

There has been an increasing recognition of the inter-relationship between human health and the oceans. Traditionally, the focus of research and concern has been on the impact of human activities on the oceans, particularly through anthropogenic pollution and the exploitation of marine resources. More recently, there has been recognition of the potential direct impact of the oceans on human health, both detrimental and beneficial. Areas identified include: global change, harmful algal blooms (HABs), microbial and chemical contamination of marine waters and seafood, and marine models and ... Read More »
» Published in Mar Pollut Bull. 2006;53(10-12):545-60. Epub 2006 Sep 25.

14. The effects of carbenoxolone on human myocardial conduction: a tool to investigate the role of gap junctional uncoupling in human arrhythmogenesis.
Match Strength: 2.413

OBJECTIVES: This study assessed the effects of carbenoxolone on human myocardial conduction and refractoriness. BACKGROUND: Carbenoxolone, an antipeptic ulcer drug, has been shown to reduce gap junctional coupling without affecting cellular ion channels. Gap junctions (GJ) are considered to be determinants of cardiac action potential propagation. The effects of GJ uncoupling in the human heart are unknown. METHODS: Right atrial (RA) and ventricular (RV) activation mapping (Carto, Biosense Webster Inc., Diamond Bar, California) was performed during sinus rhythm. Right atrial and RV wavefront ... Read More »
» Published in J Am Coll Cardiol. 2006 Sep 19;48(6):1242-9. Epub 2006 Aug 28.

15. Atypical mutational properties of human chromosome 21 suggested by comparative genome-scale analyses.
Match Strength: 2.413

Mutation of genetic material is a necessary component of evolutionary change. There is evidence for both intragenome and intergenome heterogeneity in terms of mutation frequencies. Reported comparisons of DNA sequence differences between human and chimpanzee (Pan troglodytes) suggest that human chromosome 21 may exhibit mutational hypervariability relative to the other autosomes. In the present study, further evidence is provided for such hypervariability based on large-scale analyses of amino acid composition of (translated) human genes and pseudogenes. A comparison of the variation in the ... Read More »
» Published in Cytogenet Genome Res. 2006;115(1):30-4.

16. Thrombin stimulation of p38 MAP kinase in human platelets is mediated by ADP and thromboxane A2 and inhibited by cGMP/cGMP-dependent protein kinase.
Match Strength: 2.397

p38 MAP kinase in human platelets is activated by platelet agonists including thrombin, thromboxane A2 (TxA2), ADP, and others. However, both upstream mechanisms of p38 MAP kinase activation, and their downstream sequelae, are presently controversial and essentially unclear. Certain studies report sequential activation of cGMP-dependent protein kinase (PKG) and p38/ERK pathways by platelet agonists, leading to integrin activation and secretion, whereas others establish an essential role of Src/ERK-mediated TxA2 generation for fibrinogen receptor activation in human platelets. Here, we show ... Read More »
» Published in Blood. 2007 Jan 15;109(2):616-8. Epub 2006 Sep 21.

17. A potent human anti-eotaxin1 antibody, CAT-213: isolation by phage display and in vitro and in vivo efficacy.
Match Strength: 2.346

The CC chemokine, eotaxin1 (CCL11) is an important regulator of eosinophil function. A marked accumulation of eosinophils in tissues has been correlated with the up-regulation of eotaxin1 expression in several diseases. The potential therapeutic value of neutralizing the effects of eotaxin1 in inflammatory conditions (including asthma) is under investigation. A human single-chain fragment variable antibody that neutralizes human eotaxin1 (CAT-212) was produced using antibody phage display and converted to whole antibody IgG4 format (CAT-213). A novel approach to lead optimization in which the ... Read More »
» Published in J Pharmacol Exp Ther. 2006 Dec;319(3):1395-404. Epub 2006 Sep 14.

18. Illegal use of nitrofurans in food animals: Contribution to human salmonellosis?
Match Strength: 2.341

Recent observations in Portugal of a remarkable incidence (65%) of Salmonella isolates from several sources (predominantly human and poultry) with decreased susceptibility to nitrofurantoin (MIC > or =64 mg/L), mostly comprising serogroup D isolates of Salmonella Enteritidis belonging to different phage types, suggest that illegal use of nitrofurans, especially in the poultry industry, might have contributed to the selection and prevalence of S. Enteritidis in food animals, and consequently to human salmonellosis in Portugal. Indiscriminate use of nitrofurans might also be implicated in the ... Read More »
» Published in Clin Microbiol Infect. 2006 Nov;12(11):1047-9.

19. Dioxins, polychlorinated biphenyls and other organohalogen compounds in human milk. Levels, correlations, trends and exposure through breastfeeding.
Match Strength: 2.330

Polychlorinated dibenzo-p-dioxins (PCDD) and dibenzofurans (PCDF), together simplified termed "dioxins", polychlorinated biphenyls (PCB), polybrominated diphenylethers (PBDE) and organochlorine pesticides constitute lipophilic, persistent organic pollutants that bioaccumulate in the food chain and consequently can be found in humans at considerable concentrations. During the past 30 years our institute analyzed far more than 2,000 individual human milk samples for organochlorine pesticides and PCB and over 1,000 specimens for PCDD/PCDF. The results of these analyses provide an overview and ... Read More »
» Published in Mol Nutr Food Res. 2006 Oct;50(10):922-33.

20. Laboratory investigations for the morphologic, pharmacokinetic, and anti-retroviral properties of indinavir nanoparticles in human monocyte-derived macrophages.
Match Strength: 2.326

The effectiveness of anti-retroviral therapies (ART) depends on its ultimate ability to clear reservoirs of continuous human immunodeficiency virus (HIV) infection. We reasoned that a principal vehicle for viral dissemination, the mononuclear phagocytes could also serve as an ART transporter and as such improve therapeutic indices. A nanoparticle-indinavir (NP-IDV) formulation was made and taken up into and released from vacuoles of human monocyte-derived macrophages (MDM). Following a single NP-IDV dose, drug levels within and outside MDM remained constant for 6 days without cytotoxicity. ... Read More »
» Published in Virology. 2007 Feb 5;358(1):148-58. Epub 2006 Sep 25.

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