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 << Prev 20  Showing 1 to 20 of 31 Matches Next 20 >>

1. Quasispecies and recombination.
Match Strength: 3.465

Recombination is introduced into Eigen's theory of quasispecies evolution. Comparing numerical simulations of the rate equations in the non-recombining and recombining cases show that recombination has a strong effect on the error threshold and, for a wide range of mutation rates, gives rise to two stable fixed points in the dynamics. This bi-stability results in the existence of two error thresholds. However, we prove that, for low mutation rates the bi-stability breaks down and the unique equilibrium distribution is concentrated around the sequence with highest fitness ... Read More »
» Published in Theor Popul Biol. 2006 Dec;70(4):479-85. Epub 2006 Aug 12.

2. Spontaneous attachment of amines to carbon and metallic surfaces.
Match Strength: 3.272

Primary alkylamines attach spontaneously from acetonitrile (ACN) solutions onto glassy carbon and metallic surfaces (Au, Pt, Cu, and Fe). The surface concentration of the organic layer measured from the integration of cyclic voltammograms appears close to or lower than that of a compact monolayer. The rate of the attachment depends on the concentration of the primary amino compound; it reaches a maximum after 3 h of immersion for the most concentrated solutions (20 mM). The modified surfaces have been characterized by cyclic voltammetry (CV), energy dispersive X-ray spectrometry (EDX), X-ray ... Read More »
» Published in J Phys Chem B Condens Matter Mater Surf Interfaces Biophys. 2006 Oct 5;110(39):19521-9.

3. Antibacterial principles from Myristica fragrans seeds.
Match Strength: 2.962

Nutmeg (Myristica fragrans) is used in food preparations for its aromatic flavor. The present investigation was undertaken to evaluate the antibacterial activity of constituents of M. fragrans seeds. Seeds of M. fragrans were powdered and extracted with chloroform to obtain trimyristin, which on saponification yielded myristic acid. The mother liquor remaining after separation of trimyristin was concentrated and column-chromatographed with petroleum ether to separate myristicin. Antibacterial activity of these isolated constituents was evaluated by determination of minimum inhibitory ... Read More »
» Published in J Med Food. 2006 Fall;9(3):395-9.

4. Calmodulin concentrates at the apex of growing hyphae and localizes to the Spitzenkorper in Aspergillus nidulans.
Match Strength: 2.890

The calmodulin (CaM) localization pattern in the growing hyphal tip of Aspergillus nidulans was studied with the functional GFP::CaM fusion protein. A faint tip-high gradient of CaM was found in the growing hyphal tip, with CaM highly localized in the region corresponding to the Spitzenkorper forming a bright granule. The position of highly concentrated CaM in the extreme apex seemed to determine the orientation of the hypha. The normal pattern of CaM localization was also shown to be dependent on the integrated actin cytoskeleton. When the growth of the hyphal tip ceased, CaM failed to ... Read More »
» Published in Protoplasma. 2006 Sep;228(4):159-66. Epub 2006 Sep 20.

5. PCDD/PCDF Isomer patterns in waste incinerator flyash and desorbed into the gas phase in relation to temperature.
Match Strength: 2.769

The influence of temperature on the PCDD and PCDF isomer profiles of a municipal waste incinerator flyash and the PCDD/PCDF desorbed from the ash under an inert atmosphere was investigated using a bench-scale reactor. Exposure to temperatures of 250 degrees C and above resulted in significant changes in the distribution of isomers within most dioxin and furan congener groups. The PCDD content tended to become more evenly distributed across the range of isomers than was the case for the raw flyash, while the PCDF content became concentrated across a relatively lower number of isomers. In most ... Read More »
» Published in Chemosphere. 2007 Jan;66(10):1929-38. Epub 2006 Sep 22.

6. Ca2+ and synaptotagmin VII-dependent delivery of lysosomal membrane to nascent phagosomes.
Match Strength: 2.739

Synaptotagmin (Syt) VII is a ubiquitously expressed member of the Syt family of Ca2+ sensors. It is present on lysosomes in several cell types, where it regulates Ca2+-dependent exocytosis. Because [Ca2+]i and exocytosis have been associated with phagocytosis, we investigated the phagocytic ability of macrophages from Syt VII-/- mice. Syt VII-/- macrophages phagocytose normally at low particle/cell ratios but show a progressive inhibition in particle uptake under high load conditions. Complementation with Syt VII rescues this phenotype, but only when functional Ca2+-binding sites are retained. ... Read More »
» Published in J Cell Biol. 2006 Sep 25;174(7):997-1007. Epub 2006 Sep 18.

7. Semen production in two rabbit lines divergently selected for 63-d body weight.
Match Strength: 2.579

Thirty-one bucks from two lines divergently selected for 63-d body weight (low, L and high, H) were solicited every week (twice at a 15min interval) during 18 weeks resulting in 482 ejaculates. While differing markedly on adult body weight (L: 4650g versus H: 5925g), both lines had the same testis weight. Libido did not differ between the lines. The proportion of ejaculates suitable for insemination was markedly higher in the L line (66.5% versus 44.2%). Mass motility and the volume of the ejaculates were higher in the L line while the sperm concentration was higher in the H line. Overall, the ... Read More »
» Published in Theriogenology. 2006 Dec;66(9):2165-72. Epub 2006 Sep 20.

8. HPLC-UV method for nicotine, strychnine, and aconitine in dairy products.
Match Strength: 2.546

The toxic nitrogen alkaloids nicotine, strychnine, and aconitine were quantitated in whole milk, skim milk, and cream using solid-phase extraction cleanup and HPLC-UV with dual wavelength detection. Samples were extracted in McIlvaine's buffer with EDTA and then partitioned with aqueous acetonitrile and hexane. The aqueous phase was concentrated and passed through an OASIS HLB column. The column was eluted with methylene chloride/ammonium hydroxide, 1 mL/1 microL, v/v. The eluent was acidified with hydrochloric acid and evaporated. The sample was diluted for HPLC with acetonitrile/phosphate ... Read More »
» Published in J Agric Food Chem. 2006 Oct 4;54(20):7460-5.

9. Adipophilin-enriched domains in the ER membrane are sites of lipid droplet biogenesis.
Match Strength: 2.523

The prevailing hypothesis of lipid droplet biogenesis proposes that neutral lipids accumulate within the lipid bilayer of the ER membrane from where they are budded off, enclosed by a protein-bearing phospholipid monolayer originating from the cytoplasmic leaflet of the ER membrane. We have used a variety of methods to investigate the nature of the sites of ER-lipid-droplet association in order to gain new insights into the mechanism of lipid droplet formation and growth. The three-dimensional perspectives provided by freeze-fracture electron microscopy demonstrate unequivocally that at sites ... Read More »
» Published in J Cell Sci. 2006 Oct 15;119(Pt 20):4215-24. Epub 2006 Sep 19.

10. Separation of pectin methylesterases and polygalacturonases on monolithic columns.
Match Strength: 2.465

The most abundant isoforms of tomato pectin methylesterase (PME; EC; M(r) 26kDa), polygalacturonase (PG; EC; PG1 with M(r) 82kDa) and a basic protein with M(r) 42kDa and unknown function were isolated from fresh tomato fruit by a fast chromatographic procedure on a Convective Interaction Media (CIM((R))) short monolithic disk column bearing carboxymethyl (CM) groups. The extraction of the targeted enzymes with 1.2M NaCl solution was followed by precipitation with ammonium sulfate at 60% of saturation, solubilisation of the pellet in 0.5M NaCl and fractionation using a linear ... Read More »
» Published in J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Sep 23; [Epub ahead of print]

11. Some developments in neutron and charged particle dosimetry.
Match Strength: 2.443

There is an increasing need for dosimetry of neutrons and charged particles. Increasing exposure levels are reported in the nuclear industry, deriving from more frequent in-service entries at commercial nuclear power plants, and from increased plant decommissioning and refurbishment activities. Another need stems from the compliance with requirements of the regulations and standards. The European Council directive 96/29 requires dosimetric precautions if the effective dose exceeds 1 mSv a(-1). On average, aircrew members exceed this value. Further, there is a trend of increasing use of charged ... Read More »
» Published in Radiat Prot Dosimetry. 2006;120(1-4):331-6.

12. Are liquid crystalline properties of nucleosomes involved in chromosome structure and dynamics?
Match Strength: 2.436

Nucleosome core particles correspond to the structural units of eukaryotic chromatin. They are charged colloids, 101 Angstrom in diameter and 55 Angstrom in length, formed by the coiling of a 146/147 bp DNA fragment (50 nm) around the histone protein octamer. Solutions of these particles can be concentrated, under osmotic pressure, up to the concentrations found in the nuclei of living cells. In the presence of monovalent cations (Na(+)), nucleosomes self-assemble into crystalline or liquid crystalline phases. A lamello-columnar phase is observed at 'low salt' concentrations, while a two ... Read More »
» Published in Philos Transact A Math Phys Eng Sci. 2006 Oct 15;364(1847):2615-33.

13. Accurate real-time feedback of surface EMG during fMRI.
Match Strength: 2.428

Real-time acquisition of EMG during functional MRI (fMRI) provides a novel method of controlling motor experiments in the scanner using feedback of EMG. Because of the redundancy in the human muscle system, this is not possible from recordings of joint torque and kinematics alone, because these provide no information about individual muscle activation. This is particularly critical during brain imaging because brain activations are not only related to joint torques and kinematics but are also related to individual muscle activation. However, EMG collected during imaging is corrupted by large ... Read More »
» Published in J Neurophysiol. 2007 Jan;97(1):912-20. Epub 2006 Sep 27.

14. Use of albumin in three French university hospitals: is prescription monitoring still useful in 2004?
Match Strength: 2.412

PURPOSE: Use of albumin (indications and quantities involved) has not been assessed in France since major changes occurred after the publication of Cochrane group meta-analysis. The objectives of this study were to measure the repartition of albumin indications in three French university hospitals in 2004 and to assess the feasibility and usefulness to implement a prescription-monitoring program. METHODS: Exhaustive record of albumin prescription during 2 months in three French university hospitals of Marseille. Inclusion of all patients with a first prescription of albumin between 15 March ... Read More »
» Published in Pharmacoepidemiol Drug Saf. 2007 Jan;16(1):79-85.

15. Elimination of HIV-1 infection by treatment with a doxorubicin-conjugated anti-envelope antibody.
Match Strength: 2.399

OBJECTIVE: To test the efficacy of an immunoconjugate against HIV-1. DESIGN:: A murine monoclonal antibody against the envelope antigen of HIV (P4/D10) was conjugated with the conventional anticancer drug, doxorubicin, and tested against infectious virus and infected cells, both in vitro and in vivo. METHODS: P4/D10 antibody was incubated with free virus (neutralization) or HIV-infected cells (inhibition) and the resulting infection was measured by a p24 capture enzyme-linked immunosorbent assay. In an HIV-1/MuLV murine challenge model, the ability of the conjugate to inhibit infection in vivo ... Read More »
» Published in AIDS. 2006 Oct 3;20(15):1911-5.

16. Occurrence of platinum and additional traffic related heavy metals in sediments and biota.
Match Strength: 2.395

Non-point sources play an important role in metal emissions into surface waters. One of the most important non-point sources is automobile traffic. Recent studies determining traffic related heavy metals in surface waters have concentrated mainly on worst case scenarios by analyzing heavy metal loads in waters and sediments close to storm-water overflow inlets. The present study aims at identifying traffic related heavy metals in moderately polluted sites, as they occur in highly urbanized regions. Therefore, the concentrations of eight traffic related metals (Pt, Sb, Mo, Cd, Pb, Cu, Cr and Zn ... Read More »
» Published in Chemosphere. 2007 Jan;66(4):619-29. Epub 2006 Sep 22.

17. Exploring bovine pancreatic trypsin inhibitor phase transitions.
Match Strength: 2.389

This paper presents an investigation of the phase diagram of BPTI (bovine pancreatic trypsin inhibitor)/350 mM KSCN at pH 4.9 by direct observation and numerical simulations. We report optical microscopy and light and X-ray scattering experiments coupled with theoretical data analysis using numerical tools. The phase diagram is thoroughly determined, as a function of temperature. Two polymorphs are observed by video microscopy and their solubility measured. In this phase diagram, the liquid-liquid phase separation (LLPS) is metastable with respect to the solid-liquid phase separation. Above ... Read More »
» Published in J Phys Chem B Condens Matter Mater Surf Interfaces Biophys. 2006 Oct 5;110(39):19664-70.

18. Multidetector CT (64 Slices) of the liver: examination techniques.
Match Strength: 2.359

Sixty-four-row MDCT, although developed primarily for cardiac imaging, has the potential to have a great impact on liver imaging as well. Liver-imaging protocols with sub-millimeter collimation improve longitudinal spatial resolution, making the acquired dataset a real isotropic volume perfectly designed for optimal three-dimensional rendering and accurate organ and lesion volumetry. The 64-row detector array offers a wide volumetric coverage (up to 40 mm), suitable not only for shortening scanning time and improving spatial resolution, but also for including a large volume per single rotation ... Read More »
» Published in Eur Radiol. 2006 Sep 29;

19. Integrated reverse transcription polymerase chain reaction systems for virus detection.
Match Strength: 2.355

The current study reports on an integrated microreverse transcription polymerase chain reaction (RT-PCR) system for molecular diagnosis of microorganisms automatically. By using antibodies-conjugated superparamagnetic beads, the developed system can detect viruses with higher sensitivity and specificity when compared with traditional biological diagnosis methods using a ribonucleic acid (RNA) extraction kit. The target viruses were first captured by the conjugated antibodies on the magnetic beads, and were enriched using a magnetic field generated by micro-electromagnets or permanent magnets. ... Read More »
» Published in Biosens Bioelectron. 2007 Mar 15;22(8):1739-48. Epub 2006 Sep 15.

20. Stability of nevirapine suspension in prefilled oral syringes used for reduction of mother-to-child HIV transmission.
Match Strength: 2.348

OBJECTIVE: To reduce mother-to-child transmission (MTCT) of HIV, we assessed the stability of nevirapine suspension in an oral dosing syringe over a range of storage conditions. DESIGN: A mother-to-child transmission dosing kit, containing a maternal nevirapine tablet and infant nevirapine suspension in an oral syringe that can be dispensed to the pregnant woman to use at delivery. However, the manufacturer only packages nevirapine in 240 mL, multidose containers and there are no published stability data for nevirapine suspension repackaged in an oral syringe. METHODS: Nevirapine suspension 6 ... Read More »
» Published in J Acquir Immune Defic Syndr. 2006 Nov 1;43(3):373-5.

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