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1. Duplication, divergence and formation of novel protein topologies.
Match Strength: 4.305

The rearrangement or permutation of protein substructures is an important mode of divergence. Recent work explored one possible underlying mechanism called permutation-by-duplication, which produces special forms of motif rearrangements called circular permutations. Permutation-by-duplication, involving gene duplication, fusion and truncation, can produce fully functional intermediate proteins and thus represents a feasible mechanism of protein evolution. In spite of this, circular permutations are relatively rare and we discuss possible reasons for their existence. (c) 2006 Wiley Periodicals, ... Read More »
» Published in Bioessays. 2006 Oct;28(10):973-8.

2. Production of cyclopiazonic acid, aflatrem, and aflatoxin by Aspergillus flavus is regulated by veA, a gene necessary for sclerotial formation.
Match Strength: 3.263

The plant pathogenic fungus Aspergillus flavus produces several types of mycotoxins. The most well known are the carcinogenic compounds called aflatoxins. In addition, A. flavus produces cyclopiazonic acid and aflatrem mycotoxins, contributing to the toxicity of A. flavus infected crops. Cyclopiazonic acid is a specific inhibitor of calcium-dependent ATPase in the sarcoplasmic reticulum that results in altered cellular Ca(++) levels. Aflatrem is a potent tremorgenic mycotoxin known to lead to neurological disorders. Previously we showed that a gene called veA controls aflatoxin and sclerotial ... Read More »
» Published in Appl Microbiol Biotechnol. 2007 Jan;73(5):1158-68. Epub 2006 Sep 19.

3. Absorption and emission spectroscopic characterization of blue-light receptor Slr1694 from Synechocystis sp. PCC6803.
Match Strength: 3.178

The BLUF protein Slr1694 from the cyanobacterium Synechocystis sp. PCC6803 is characterized by absorption and emission spectroscopy. Slr1694 expressed from E. coli which non-covalently binds FAD, FMN, and riboflavin (called Slr1694(I)), and reconstituted Slr1694 which dominantly contains FAD (called Slr1694(II)) are investigated. The receptor conformation of Slr1694 (dark adapted form Slr1694(r)) is transformed to the putative signalling state (light adapted form Slr1694(s)) with red-shifted absorption and decreased fluorescence efficiency by blue-light excitation. In the dark at 22 degrees C, ... Read More »
» Published in J Photochem Photobiol B. 2007 Jan 3;86(1):22-34. Epub 2006 Sep 22.

4. Sequence identity of the direct repeats, DR1 and DR2, contributes to the discrimination between primer translocation and in situ priming during replication of the duck hepatitis B virus.
Match Strength: 2.996

There are two mutually exclusive pathways for plus-strand DNA synthesis in hepadnavirus reverse transcription. The predominant pathway gives rise to relaxed circular DNA, while the other pathway yields duplex linear DNA. At the completion of minus-strand DNA synthesis, the final RNase H cleavage generates the plus-strand primer at direct repeat 1 (DR1). A small fraction of viruses make duplex linear DNA after initiating plus-strand DNA synthesis from this site, a process called in situ priming. To make relaxed circular DNA, a template switch is necessary for the RNA primer generated at DR1 to ... Read More »
» Published in J Mol Biol. 2006 Nov 17;364(1):32-43. Epub 2006 Sep 7.

5. Cryopreservation of the ovary by vitrification as an alternative to slow-cooling protocols.
Match Strength: 2.785

OBJECTIVE: To study the thermal properties of a cryoprotectant solution, called VS4, and of VS4-impregnated whole sheep ovaries with pedicle. DESIGN: Physical and experimental animal study. SETTING: Academic research environment. ANIMAL(S): Five- to 6-month-old ewes. INTERVENTION(S): Thermal properties on cooling of a cryoprotectant solution called VS4 were measured by differential scanning calorimetry. VS4 contains 2.75 mol/L dimethyl sulfoxide, 2.76 mol/L formamide, and 1.97 mol/L propylene glycol. Whole sheep ovaries were collected at the slaughterhouse and prepared for a vitrification ... Read More »
» Published in Fertil Steril. 2006 Oct;86 Suppl 4:1243-51. Epub 2006 Sep 14.

6. Are desmoglein autoantibodies essential for the immunopathogenesis of pemphigus vulgaris, or just "witnesses of disease"?
Match Strength: 2.652

Pemphigus vulgaris (PV) is fascinating to dermatologists, epithelial biologists and immunologists alike, as its pathogenesis has been clarified to a much greater extent than that of most other organ-specific autoimmune diseases, and as it has provided abundant novel insights into desmoglein biology and pathology along the way. Historically, the most influential PV pathogenesis concept is that of Stanley and Amagai. This concept holds that autoantibodies against desmogleins are both essential and sufficient for epidermal blister formation (acantholysis) by impeding the normal functioning of ... Read More »
» Published in Exp Dermatol. 2006 Oct;15(10):815-31. Erratum in: Exp Dermatol. 2006 Dec;15(12):1016. Kurzen, H [added].

7. Near-field microscopy through a SiC superlens.
Match Strength: 2.635

The wave nature of light limits the spatial resolution in classical microscopy to about half of the illumination wavelength. Recently, a new approach capable of achieving subwavelength spatial resolution, called superlensing, was invented, challenging the already established method of scanning near-field optical microscopy (SNOM). We combine the advantages of both techniques and demonstrate a novel imaging system where the objects no longer need to be in close proximity to a near-field probe, allowing for optical near-field microscopy of subsurface objects at sub-wavelength-scale lateral ... Read More »
» Published in Science. 2006 Sep 15;313(5793):1595.

8. Pathway classification of TCA cycle.
Match Strength: 2.630

The structural analysis of large metabolic networks exhibits a combinatorial explosion of elementary modes. A new method of classification has been developed [called aggregation around common motif (ACoM)], which groups elementary modes into classes with similar substructures. This method is applied to the tricarboxylic acid cycle and metabolite carriers. The analysis of this network evidences a great number of elementary flux modes (204) despite the low number of reactions (23). The ACoM is used to class these elementary modes in a low number of sets (8) with biological meanings ... Read More »
» Published in Syst Biol (Stevenage). 2006 Sep;153(5):369-71.

9. Mature teratoma presenting as a scalp mass in a newborn.
Match Strength: 2.623

Neonatal teratomas are rarely located in the scalp. We present a 10-day-old female newborn with mature teratoma of the occipital scalp. The tumor mass, which had no intracranial extension, was excised completely when the patient was 14 days old. The scalp defect was reconstructed with local flaps. No recurrence was detected 3 months after the surgery. Because the patient did not return for routine follow-up 6 months after surgery, we called the parents and learned that the patient had suddenly died. A necropsy to explain the cause of death was not available. Publication Types: Case ... Read More »
» Published in J Craniofac Surg. 2006 Sep;17(5):1009-11.

10. An extended likelihood ratio framework for interpreting evidence.
Match Strength: 2.619

This paper reviews some current methods, the likelihood ratio-based approach and the full Bayesian approach for the interpretation of evidence and discusses previously identified shortcomings in them. It suggests an approach based on a compromise--based on an extended likelihood ratio--that may combine the merits of logic without overstepping acceptable bounds for the forensic scientist in the presentation of evidence. The approach is exposed formally and takes advantage of inferential networks called Bayesian networks ... Read More »
» Published in Sci Justice. 2006 Apr-Jun;46(2):69-78.

11. Tool kit for the staff mentor: strategies for improving retention.
Match Strength: 2.590

Retention of new graduates in nursing continues to be a significant workplace issue, particularly in light of the heightening nursing shortage. Experienced nurses are in a pivotal position to positively impact retention, and, because of this, they can be called "keystoners". Mentoring, an important role of the keystoner, requires specific skills and knowledge and can be learned. A tool kit designed to develop the mentoring skills of keystoners is described. The result of successful mentoring among keystoners can be multifaceted; workplace satisfaction and higher retention of new graduates are ... Read More »
» Published in J Contin Educ Nurs. 2006 Sep-Oct;37(5):210-3.

12. Echocardiographic evaluation of mitral valve repair by modified "edge to edge" technique.
Match Strength: 2.535

The "edge to edge" or "double-orifice" technique is an alternative surgical option of mitral valve repair to treat mitral regurgitation. Echocardiography is very useful to evaluate the postoperative valve function, but since this technique is not frequently used, there is little experience about its echocardiographic features, which are different from those of the classic mitral valve repair. In this report, we present a patient who underwent this repair with a modified approach called "triple-orifice technique" and was evaluated by transthoracic and transesophageal echocardiography ... Read More »
» Published in Echocardiography. 2006 Oct;23(9):771-3.

13. The exocytoskeleton.
Match Strength: 2.454

The murein or peptidoglycan wall enclosing most bacteria is essential for the life style of most organisms in the Domain of Bacteria. Only in special situations does it not play a role in the bacterial growth cycle. When life first appeared on this planet the cellular osmotic pressure was probably low and a sacculus was probably not relevant, but became necessary as bacterial life evolved from the complex and sophisticated cell called the Last Universal Ancestor. The construction of the murein wall outside of the cytoplasmic membrane is complex and requires elaborate special biochemistry. ... Read More »
» Published in J Mol Microbiol Biotechnol. 2006;11(3-5):115-25.

14. Inequalities in oncology care: Economic consequences of high cost drugs.
Match Strength: 2.422

The expenditures for hospital drugs are continuously increasing, and grow much faster than the global hospital budgets do. This explosive growth is caused mainly by a few so-called 'expensive drugs' of which the oncolytics form the main part. The global budgets should stimulate more effective provision of care ('technical efficiency'), however the room for technical efficiency is decreasing. Hospitals thus have to make impossible choices, so that eventually equal access can no longer be guaranteed. If no other policies are applied, health care goals will no longer be met. This paper tries to ... Read More »
» Published in Eur J Cancer. 2006 Nov;42(17):2887-92. Epub 2006 Sep 18.

15. Inversion mode display of 3D sonography: applications in obstetric and gynecologic imaging.
Match Strength: 2.414

OBJECTIVE: Three-dimensional sonography involves volume acquisitions of sonographic data that can be displayed in a variety of ways, including surface rendering and multiplanar reconstruction. A new method of displaying sonographic volumes is called the inversion mode, which displays the cystic portions within the entire volume as echogenic areas. The gray-scale portion of the image becomes transparent, and the cystic areas become brightly visible in three dimensions. CONCLUSION: This article shows the applications for this method of volume sonography display and shows the importance of being ... Read More »
» Published in AJR Am J Roentgenol. 2006 Oct;187(4):965-71.

16. The genetic conception of health: is it as radical as claimed?
Match Strength: 2.373

The so-called new genetics is widely predicted to radically transform medicine and public health and deliver considerable benefits in the future. This article argues that, although it is doubtful that many of the promised benefits of genetic research will be delivered, an increasingly pervasive genetic worldview and expectations about future genetic innovations are profoundly shaping conceptions of health and illness and priorities in healthcare. Further, it suggests that debates about the normative and justice implications of new genetic technologies thus far have been constrained by ... Read More »
» Published in Health (London). 2006 Oct;10(4):481-500.

17. Transition of ovalbumin to thermostable structure entails conformational changes involving the reactive center loop.
Match Strength: 2.278

Ovalbumin is a serpin without inhibitory activity against proteases. During embryonic development, ovalbumin in the native (N) form undergoes changes and takes a heat-stable form, which was previously named HS-ovalbumin. It has been known that N-ovalbumin is artificially converted to another thermostable form called S-ovalbumin by heating at an alkaline pH. Here, we characterized further the three ovalbumin forms, N, HS, and S. The epitope of the monoclonal antibody 2B3/2H11, which recognizes N- and HS-ovalbumin but not S-ovalbumin, was found to reside in the region Glu-Val-Val-Gly-Ala-Ser-Glu ... Read More »
» Published in Biochim Biophys Acta. 2007 Jan;1770(1):5-11. Epub 2006 Jul 25.

18. Correlation of norovirus variants with epidemics of acute viral gastroenteritis in Hong Kong.
Match Strength: 2.278

Norovirus (NV) (formerly called Norwalk-like virus) is the most common etiological agent of acute viral gastroenteritis outbreaks worldwide. Recent reports have shown that two new GII.4 variants caused epidemics in Europe. To investigate if it is also the case in Hong Kong, a molecular epidemiological study was undertaken between January 2002 and June 2005. During this period, there was a substantial increase in acute cases of gastroenteritis caused by NV. Phylogenetic analysis showed that GII.2 and GII.4 are the major circulating genotypes. Two new GII.4 variants (variants C and D) were ... Read More »
» Published in J Med Virol. 2006 Nov;78(11):1473-9.

19. 'Genetics home reference': helping patients understand the role of genetics in health and disease.
Match Strength: 2.183

The surge of information generated by the Human Genome Project has left many health professionals and their patients struggling to understand the role of genetics in health and disease. To aid the lay public and health professionals, the US National Library of Medicine developed an online resource called 'Genetics Home Reference' (GHR), located at http://ghr.nlm.nih.gov/. Launched in April 2003, GHR's goal is to help the public interpret the health implications of the Human Genome Project. It bridges the clinical questions of consumers and the rich technical data emerging from the sequenced ... Read More »
» Published in Community Genet. 2006;9(4):274-8.

20. The Contaminated High-Energy Open Fracture: A Protocol to Prevent and Treat Inflammatory Mediator Storm-Induced Soft-Tissue Compartment Syndrome (IMSICS).
Match Strength: 2.182

The treatment modalities currently used in surgical debridement leave the traumatic wound with viable but tenuous tissue and a variable level of microcontaminants potentially laden with bacteria. In high-energy contaminated wounds, retention of these contaminants within the tenuous tissue of the so-called zone of stasis can result in further tissue necrosis and the development of infection. A novel protocol for managing the high-energy contaminated open fracture involves two new techniques. First, Bernoulli's principle is used to facilitate a systematic excision of contaminants, as well as the ... Read More »
» Published in J Am Acad Orthop Surg. 2006 Oct;14(10 Suppl):S82-6.

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