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1. Astrocyte calcium elevations: properties, propagation, and effects on brain signaling.
Match Strength: 5.191

The possibility that astrocytes are involved in brain signaling began to emerge in the late 1970s, when it was first shown that astroglia in vitro possess numerous receptors for neurotransmitters. It was later demonstrated that cultured astroglia and astrocytes in situ respond to neurotransmitters with increases in intracellular second messengers, including cyclic AMP and calcium. Astrocyte calcium responses have since been extensively studied both in culture and in intact tissue. We continue to gather information regarding the various compounds able to trigger astrocyte calcium increases, as ... Read More »
» Published in Glia. 2006 Nov 15;54(7):676-90.

2. Effect of dietary calcium intake on weight gain in type 2 diabetic patients following initiation of insulin therapy.
Match Strength: 5.019

OBJECTIVES: This pilot study analyses weight gain in type 2 diabetic patients at initiation of insulin therapy, according to daily calcium intake. METHODS: Type 2 diabetic patients consecutively admitted for initiation of insulin therapy were studied between January and March 2004 in a monocenter study. Dietary intake was assessed by a 7-day food history before insulin treatment (initial visit) and 4 to 6 months later (final visit). RESULTS: Thirty-one patients were studied (18 males and 13 females; mean age 62+/-9 years, with diabetes duration 14+/-10 years). Weight significantly increased ... Read More »
» Published in Diabetes Metab. 2006 Sep;32(4):358-63.

3. Ingestion of difructose anhydride III, a non-digestible disaccharide, improves postgastrectomy osteopenia in rats.
Match Strength: 4.997

OBJECTIVE: Total gastrectomy produces osteopenia with calcium malabsorption. We previously demonstrated that difructose anhydride III (DFAIII), a non-digestible disaccharide, stimulates intestinal calcium absorption in normal and ovariectomized rats. In the present study, we examined the effects of feeding DFAIII on gastrectomy-induced calcium malabsorption and osteopenia in rats. The potential of DFAIII to promote large intestinal calcium absorption was also evaluated through comparison with that of fructooligosaccharides (FOS). MATERIAL AND METHODS: Male Sprague-Dawley rats were divided into ... Read More »
» Published in Scand J Gastroenterol. 2006 Oct;41(10):1165-73.

4. Intracellular Ca2+ imaging in C. elegans.
Match Strength: 4.862

Optical methods provide a noninvasive way to monitor calcium transients in Caenorhabditis elegans. Imaging techniques are particularly appealing in C. elegans because worms are optically transparent and can be imaged while fully intact. Furthermore, a variety of genetically encoded calcium indicators are available that can be targeted to cells of interest with appropriate tissue-specific promoters. Here, we describe a specific protocol, suitable for monitoring neuronal activity, for rapid calcium imaging in C. elegans using the cameleon indicator. Notes are provided to assist with adapting ... Read More »
» Published in Methods Mol Biol. 2006;351:253-64.

5. Multiple glycerol shocks increase the calcium phosphate transfection of non-synchronized CHO cells.
Match Strength: 4.801

The exposure of CHO DG44 cells to an osmotic shock, after DNA uptake, results in a cellular volume decrease of approx. 55%. Repetitive osmotic shocks targeted different sub-populations of cells as was demonstrated using two different fluorescent reporter genes. Also the exposure of a calcium phosphate-DNA coprecipitate to high osmolarity in vitro caused the release of the DNA from the precipitate. The results demonstrate the importance of the osmotic shock on the efficient delivery of plasmid DNA to the nucleus of CHO cells following calcium phosphate-mediated transfection ... Read More »
» Published in Biotechnol Lett. 2006 Nov;28(22):1827-33. Epub 2006 Sep 29.

6. Quantification and characterization of carotid calcium with multi-detector CT-angiography.
Match Strength: 4.456

OBJECTIVE: The aim of this study was to assess the accuracy of CT-angiography for identification and measurement of calcification of carotid atherosclerotic plaques and to characterise the content and distribution pattern of mineral calcium (hydroxyapatite, Ca) in carotid bifurcations and investigate its relationship with neurological symptoms. METHODS: Twenty-six patients with ICA stenosis > 60% (13 symptomatic, 13 asymptomatic) were selected for study. Ca was estimated from the weight of the ashed remnants of carotid endarterectomy (CEA) specimens in 11 patients. Calcium content ... Read More »
» Published in Eur J Vasc Endovasc Surg. 2006 Nov;32(5):561-7. Epub 2006 Sep 18.

7. Rapid transcriptome changes induced by cytosolic Ca2+ transients reveal ABRE-related sequences as Ca2+-responsive cis elements in Arabidopsis.
Match Strength: 4.433

The regulation of gene expression by cellular calcium is crucial for plant defense against biotic and abiotic stresses. However, the number of genes known to respond to specific transient calcium signals is limited, and as yet there is no definition of a calcium-responsive cis element in plants. Here, we generated specific cytosolic calcium transients in intact Arabidopsis thaliana seedlings and linked them to early transcriptome changes, followed by bioinformatic analysis of the responsive genes. A cytosolic calcium transient induced by calmodulin antagonists and blocked by lanthanides was ... Read More »
» Published in Plant Cell. 2006 Oct;18(10):2733-48. Epub 2006 Sep 15.

8. Pseudomonas aeruginosa autoinducer modulates host cell responses through calcium signalling.
Match Strength: 4.213

The opportunistic pathogen Pseudomonas aeruginosa utilizes a cell density-dependent signalling phenomenon known as quorum sensing (QS) to regulate several virulence factors needed for infection. Acylated homoserine lactones, or autoinducers, are the primary signal molecules that mediate QS in P. aeruginosa. The autoinducer N-3O-dodecanoyl-homoserine lactone (3O-C12) exerts effects on mammalian cells, including upregulation of pro-inflammatory mediators and induction of apoptosis. However, the mechanism(s) by which 3O-C12 affects mammalian cell responses is unknown. Here we report that 3O-C12 ... Read More »
» Published in Cell Microbiol. 2006 Oct;8(10):1601-10.

9. Effects of calcium supplementation on bone density in healthy children: meta-analysis of randomised controlled trials.
Match Strength: 4.151

OBJECTIVES: To assess the effectiveness of calcium supplementation for improving bone mineral density in healthy children and to determine if any effect is modified by other factors and persists after supplementation stops. DESIGN: Meta-analysis. DATA SOURCES: Electronic bibliographic databases, hand searching of conference proceedings, and contacting authors for unpublished data. REVIEW METHODS: We included randomised placebo controlled trials of calcium supplementation in healthy children that lasted at least three months and had bone outcomes measured after at least six months of follow-up. ... Read More »
» Published in BMJ. 2006 Oct 14;333(7572):775. Epub 2006 Sep 15. Comment in: BMJ. 2006 Oct 14;333(7572):763-4.

10. Renal tubular acidosis type 2 with Fanconi's syndrome, osteomalacia, osteoporosis, and secondary hyperaldosteronism in an adult consequent to vitamin D and calcium deficiency: effect of vitamin D and calcium citrate therapy.
Match Strength: 4.079

OBJECTIVE: To describe a unique example of renal tubular acidosis type 2 (RTA 2) in conjunction with Fanconi's syndrome and osteomalacia consequent to vitamin D and calcium deficiency in an adult without underlying gastrointestinal disease. METHODS: We review the clinical, hormonal, histomorphometric, and micro-computed tomographic findings and the response to therapy with vitamin D and calcium in our patient. RESULTS: On admission, a 33-year-old African American woman had the following laboratory findings: serum ionized calcium 3.8 mg/dL (0.95 mmol/L), venous pH 7.26, bicarbonate 20 mEq/L, ... Read More »
» Published in Endocr Pract. 2006 Sep-Oct;12(5):559-67.

11. Dietary calcium and magnesium, major food sources, and risk of type 2 diabetes in U.S. black women.
Match Strength: 4.035

OBJECTIVE: Inverse associations between magnesium and calcium intakes and risk of type 2 diabetes have been reported for studies in predominantly white populations. We examined magnesium, calcium, and major food sources in relation to type 2 diabetes in African-American women. RESEARCH DESIGN AND METHODS: This is a prospective cohort study including 41,186 participants of the Black Women's Health Study without a history of diabetes who completed validated food frequency questionnaires at baseline. During 8 years of follow-up (1995-2003), we documented 1,964 newly diagnosed cases of type 2 ... Read More »
» Published in Diabetes Care. 2006 Oct;29(10):2238-43.

12. Sigma-1 receptor activation prevents intracellular calcium dysregulation in cortical neurons during in vitro ischemia.
Match Strength: 3.831

Sigma receptors are putative targets for neuroprotection following ischemia; however, little is known on their mechanism of action. One of the key components in the demise of neurons following ischemic injury is the disruption of intracellular calcium homeostasis. Fluorometric calcium imaging was used to examine the effects of sigma receptor activation on changes in intracellular calcium concentrations ([Ca(2+)](i)) evoked by in vitro ischemia in cultured cortical neurons from embryonic rats. The sigma receptor agonist, 1,3-di-o-tolyl-guanidine (DTG), was shown to depress [Ca(2+)](i) ... Read More »
» Published in J Pharmacol Exp Ther. 2006 Dec;319(3):1355-65. Epub 2006 Sep 20.

13. mRNA and protein expression and activities of nitric oxide synthases in the lumbar spinal cord of neonatal rats after sciatic nerve transection and melatonin administration.
Match Strength: 3.829

Sciatic axotomy in 2-day-old rats (P2) causes lumbar motoneuron loss, which could be associated with nitric oxide (NO) production. NO may be produced by three isoforms of synthase (NOS): neuronal (nNOS), endothelial (eNOS) and inducible (iNOS). We investigated NOS expression and NO synthesis in the lumbar enlargement of rats after sciatic nerve transection at P2 and treatment with the antioxidant melatonin (sc; 1 mg/kg). At time points ranging from P2 to P7, expression of each isoform was assessed by RT-PCR and immunohistochemistry; catalytic rates of calcium-dependent (nNOS, eNOS) and ... Read More »
» Published in Neurosci Lett. 2006 Oct 23;407(2):182-7. Epub 2006 Sep 15.

14. Factors affecting the structure and properties of an injectable self-setting calcium phosphate foam.
Match Strength: 3.810

One of the main challenges in the investigation on calcium phosphate cements (CPC) lies in the introduction of macroporosity, without loosing the self-setting ability and injectability, characteristic of the cement-type materials. The benefits of macroporosity are related to the enhancement of bone regeneration mechanisms, such as angiogenesis and tissue ingrowth. In this work, the feasibility to obtain self-setting injectable macroporous hydroxyapatite foams by the incorporation of a protein-based foaming agent to a CPC is demonstrated. Albumen is combined with an alpha-tricalcium phosphate ... Read More »
» Published in J Biomed Mater Res A. 2007 Feb;80(2):351-61.

15. Metabolic regulation of sodium-calcium exchange by intracellular acyl CoAs.
Match Strength: 3.804

The sodium-calcium exchanger (NCX) is a critical mediator of calcium homeostasis. In the heart, NCX1 predominantly operates in forward mode to extrude Ca(2+); however, reverse-mode NCX1 activity during ischemia/reperfusion (IR) contributes to Ca(2+) loading and electrical and contractile dysfunction. IR injury has also been associated with altered fat metabolism and accumulation of long-chain acyl CoA esters. Here, we show that acyl CoAs are novel, endogenous activators of reverse-mode NCX1 activity, exhibiting chain length and saturation dependence, with longer chain saturated acyl moieties ... Read More »
» Published in EMBO J. 2006 Oct 4;25(19):4605-14. Epub 2006 Sep 14.

16. Novel highly biodegradable biphasic tricalcium phosphates composed of alpha-tricalcium phosphate and beta-tricalcium phosphate.
Match Strength: 3.721

Novel biodegradable biphasic tricalcium phosphates (BTCP) composed of alpha-tricalcium phosphate (alpha-TCP) and beta-tricalcium phosphate (beta-TCP) were successfully synthesized by heating amorphous calcium phosphate precursors with different structures at 800 degrees C for 3h. The ratio of alpha-TCP and beta-TCP in the calcium phosphate particle can be controlled by aging time and pH value during synthesis of the amorphous precursor ... Read More »
» Published in Acta Biomater. 2006 Sep 14;

17. Astrocyte calcium waves: what they are and what they do.
Match Strength: 3.668

Several lines of evidence indicate that the elaborated calcium signals and the occurrence of calcium waves in astrocytes provide these cells with a specific form of excitability. The identification of the cellular and molecular steps involved in the triggering and transmission of Ca(2+) waves between astrocytes resulted in the identification of two pathways mediating this form of intercellular communication. One of them involves the direct communication between the cytosols of two adjoining cells through gap junction channels, while the other depends upon the release of "gliotransmitters" that ... Read More »
» Published in Glia. 2006 Nov 15;54(7):716-25.

18. 'Trigger' events precede calcium puffs in Xenopus oocytes.
Match Strength: 3.663

The liberation of calcium ions sequestered in the endoplasmic reticulum through inositol 1,4,5-trisphosphate receptors/channels (IP(3)Rs) results in a spatiotemporal hierarchy of calcium signaling events that range from single-channel openings to local Ca(2+) puffs believed to arise from several to tens of clustered IP(3)Rs to global calcium waves. Using high-resolution confocal linescan imaging and a sensitive Ca(2+) indicator dye (fluo-4-dextran), we show that puffs are often preceded by small, transient Ca(2+) elevations that we christen "trigger events". The magnitude of triggers is ... Read More »
» Published in Biophys J. 2006 Dec 1;91(11):4024-32. Epub 2006 Sep 15.

19. The therapeutic potential of the calpain family: new aspects.
Match Strength: 3.661

The calpain family is a group of cysteine proteases unique in their dependency on calcium to attain functionally active forms. Calpains are involved in a wide range of cellular calcium-regulated functions, including signal transduction, cell proliferation and differentiation, and apoptosis. Moreover, altered calpain activity has been observed in several human diseases. Specific calpain inhibitors hold promise for the treatment of neuromuscular and neurodegenerative diseases in which calpains have been shown to be upregulated (e.g. Parkinson's disease and Duchenne muscular dystrophy). ... Read More »
» Published in Drug Discov Today. 2006 Oct;11(19-20):917-23. Epub 2006 Sep 7.

20. Activation of the extracellular calcium-sensing receptor initiates insulin secretion from human islets of Langerhans: involvement of protein kinases.
Match Strength: 3.600

The extracellular calcium-sensing receptor (CaR) is usually associated with systemic Ca(2+) homeostasis, but the CaR is also expressed in many other tissues, including pancreatic islets of Langerhans. In the present study, we have used human islets and an insulin-secreting cell line (MIN6) to investigate the effects of CaR activation using the calcimimetic R-568, a CaR agonist that activates the CaR at physiological concentrations of extracellular Ca(2+). CaR activation initiated a marked but transient insulin secretory response from both human islets and MIN6 cells at a sub-stimulatory ... Read More »
» Published in J Endocrinol. 2006 Sep;190(3):703-10.

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