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Human Retrovirus
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1. Insertional polymorphism of endogenous retrovirus HERV-K115 in schizophrenia.
Match Strength: 8.532

Retroviruses are implicated in the pathogenesis of schizophrenia. Human endogenous retrovirus type K115 (HERV-K115) is a full-length, potentially transcriptional retrovirus and is also polymorphic. We investigated the frequency of HERV-K115 in Japanese schizophrenia patients and healthy controls. No difference was found in the frequency between patients and controls (8.4% versus 9.4%, respectively). However, a marginal difference was observed in age at onset between the HERV-K positive and negative patients (p=0.057). The HERV-K115 insertion appeared to be more frequent in patients with ... Read More »
» Published in Neurosci Lett. 2006 Nov 20;408(3):226-9. Epub 2006 Sep 26.

2. In vivo screening of porcine endogenous retrovirus in Chinese Banna minipig inbred.
Match Strength: 7.956

The risk of porcine endogenous retrovirus (PERV) infection is one of the major barriers in clinical trials of pig-to-human xenotransplantation. Previous experiments showed that PERV could infect many types of human and nonhuman primate cells, but there is no reported evidence of in vivo infection. In this study, extracted genomic DNA from tissues of seventeen pigs was analyzed using specific sequence primers for gag, pol, and env. The results suggested that PERV exist in the genomes of all tissues. A subtype analysis indicated that PERV-A and PERV-B were in the tissue genome with no positive ... Read More »
» Published in Transplant Proc. 2006 Sep;38(7):2261-3.

3. Modulating target site selection during human immunodeficiency virus DNA integration in vitro with an engineered tethering factor.
Match Strength: 4.353

The mechanisms controlling retroviral integration have been the topic of intense interest, in part because of adverse clinical events that occurred during retrovirus-mediated human gene therapy. Here we investigate the use of artificial tethering interactions to constrain retroviral integration site selection in an in vitro model. During normal infection, HIV DNA integration is favored in active cellular transcription units. One component of the targeting mechanism is the cellular LEDGF/p75 protein. LEDGF/p75 binds tightly to HIV integrase (IN) protein, and depletion of LEDGF/p75 from target ... Read More »
» Published in Hum Gene Ther. 2006 Sep;17(9):960-7.

4. Transduction of human embryonic stem cells by ecotropic retroviral vectors.
Match Strength: 4.216

The steadily increasing availability of human embryonic stem (hES) cell lines has created strong interest in applying available tools for gene transfer in murine cells to human systems. Here we present a method for the transduction of hES cells with ecotropic retroviral vectors. hES cells were transiently transfected with a construct carrying the murine retrovirus receptor mCAT1. Subsequently, the cells were exposed to replication-deficient Moloney murine leukemia virus (MoMuLV) derivatives or pseudotyped lentiviral vectors. With oncoretroviral vectors, this procedure yields overall ... Read More »
» Published in Nucleic Acids Res. 2006;34(18):e120. Epub 2006 Sep 22.

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