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1. Pneumocystis: newer knowledge about the biology of this group of organisms in laboratory rats and mice.
Match Strength: 5.419

This review is an update on some of the remarkable advances that have led to greater understanding of Pneumocystis, an important group of rodent pathogens. The author outlines the life cycle of these pulmonic fungi, their taxonomic position, and their nomenclature. He discusses how infections begin and spread in laboratory rodent colonies, and how those infections are inadvertently maintained in barriered breeding colonies. He also addresses the diagnosis of Pneumocystis infection and provides suggestions for the establishment of Pneumocystis-free animal colonies. Publication Types: ... Read More »
» Published in Lab Anim (NY). 2006 Oct;35(9):55-61.

2. Sporotrichosis: the main differential diagnosis with tegumentary leishmaniosis in dogs from Rio de Janeiro, Brazil.
Match Strength: 4.930

Seventy-four dogs from the State of Rio de Janeiro with ulcerated cutaneous lesions were submitted to clinical, dermatological, parasitological, mycological, histopathological and cytopathological exams, a leishmanin skin test, an indirect immunofluorescence (IIF) test for leishmaniosis, and nonspecific laboratory tests such as blood count and serum biochemistry. Sporothrix schenckii was isolated from 41 dogs and Leishmania (Viannia) braziliensis was isolated from 33 animals. Most dogs with sporotrichosis were from the municipality of Rio de Janeiro (53.7%) and presented ulcerated cutaneous ... Read More »
» Published in Vet Parasitol. 2007 Jan 19;143(1):1-6. Epub 2006 Sep 12.

3. Discrepancy in the use of confirmatory tests in patients hospitalized with the diagnosis of chronic obstructive pulmonary disease or congestive heart failure.
Match Strength: 4.917

OBJECTIVE: To determine the prevalence of confirmatory use of spirometry in patients admitted to a tertiary-care facility with the diagnosis of chronic obstructive pulmonary disease (COPD), including those with respiratory failure, and compare that to the use of confirmatory 2-dimensional echocardiography (2-D echo) in patients admitted with the diagnosis of congestive heart failure (CHF), to determine preferential confirmatory testing practices. SETTING: Academic tertiary-care hospital. METHODS: A 6-month retrospective review of charts of patients with a primary or secondary discharge ... Read More »
» Published in Respir Care. 2006 Oct;51(10):1120-4. Comment in: Respir Care. 2006 Oct;51(10):1116-7.

4. Leptospirosis in Istanbul, Turkey: a wide spectrum in clinical course and complications.
Match Strength: 4.716

Patients with high fever and multiorgan involvement were investigated for the determination of frequency, clinical course and complications of leptospirosis in Istanbul. Leptospirosis was determined in 22 cases among the 35 hospitalized patients that were pre-diagnosed as leptospirosis according to 'Probable Leptospirosis Diagnosis and Follow-up' form. Among the leptospirosis cases 19 were male and 16 were military staff. Mean age was 35.6 y. Dark field examination (DFE), latex agglutination test (LAG), ELISA IgM, leptospirosis culture (LC) and microscopic agglutination test (MAT) were ... Read More »
» Published in Scand J Infect Dis. 2006;38(10):845-52.

5. A case of progressive pseudorheumatoid arthropathy of 'childhood' with the diagnosis delayed to the fifth decade.
Match Strength: 4.530

Progressive pseudorheumatoid arthropathy of childhood (PPAC) is a rare single gene disorder which is frequently misdiagnosed as juvenile rheumatoid arthritis. It is characterised with arthralgia, joint contractures, bony swelling of metacarpophalangeal and interphalangeal joints and platyspondyly. Clinical and laboratory signs of joint inflammation such as synovitis, a high erythrocyte sedimentation rate and an elevated C-reactive protein level are usually absent. Although the disease begins early in life (usually between 3 and 8 years of age), the diagnosis may be delayed. In the present case ... Read More »
» Published in Int J Clin Pract. 2006 Oct;60(10):1306-9.

6. Therapeutic aspects of fat embolism syndrome.
Match Strength: 4.235

Signs and symptoms of clinical fat embolism syndrome (FES) usually begin within 24-48h after trauma. The classic triad involves pulmonary changes, cerebral dysfunction, and petechial rash. Clinical diagnosis is key because laboratory and radiographic diagnosis is not specific and can be inconsistent. The duration of FES is difficult to predict because it is often subclinical or may be overshadowed by other illnesses or injuries. Medical care is prophylactic or supportive, including early fixation and general ICU management to ensure adequate oxygenation and ventilation, hemodynamic stability, ... Read More »
» Published in Injury. 2006 Oct;37 Suppl 4:S68-73.

7. Thoracic empyema in patients with community-acquired pneumonia.
Match Strength: 4.199

PURPOSE: The objective of this study was to update the incidence and natural history of empyema in patients admitted to hospital with community-acquired pneumonia (CAP). METHODS: A prospective population-based study of 3675 patients with a diagnosis of CAP was carried out. Patients were classified as "definite empyema" based on one or more of the following criteria: presence of microorganisms in pleural fluid Gram stain or culture, pleural pH less than 7.2 associated with radiographic features of empyema, or frank pus in the pleural space at the time of thoroscopy. All others were classified ... Read More »
» Published in Am J Med. 2006 Oct;119(10):877-83.

8. Utility of plasma free metanephrines in diagnosis of factitious pheochromocytoma.
Match Strength: 3.945

OBJECTIVE: To report a case of epinephrine-induced factitious pheochromocytoma in a young woman with a past medical history of Conn's syndrome. METHODS: We present a case report with clinical and laboratory details, review related reports in the literature, and demonstrate the usefulness of plasma free metanephrine levels in facilitating the diagnosis of factitious pheochromocytoma. RESULTS: A 34-year-old woman was admitted to our hospital for confirmation and localization of an occult pheochromocytoma. After thorough investigation, we discovered that the patient was surreptitiously injecting ... Read More »
» Published in Endocr Pract. 2006 Sep-Oct;12(5):568-71.

9. Immunohistochemistry using a Mycobacterium tuberculosis complex specific antibody for improved diagnosis of tuberculous lymphadenitis.
Match Strength: 3.894

The clinical and histological criteria used to diagnose lymphadenitis caused by Mycobacterium tuberculosis complex organisms have poor specificity. Acid-fast staining and culture has low sensitivity and specificity. We report a novel method for diagnosis of tuberculosis that uses immunohistochemistry to detect the secreted mycobacterial antigen MPT64 on formalin-fixed tissue biopsies. This antigen has not been detected in non-tuberculous mycobacteria. Polymerase chain reaction (PCR) for amplification of IS6110 from DNA obtained from the biopsies was used as a gold standard. Fifty-five cases of ... Read More »
» Published in Mod Pathol. 2006 Dec;19(12):1606-14. Epub 2006 Sep 15.

10. Traumatic fracture in a healthy man: benign or pathologic?
Match Strength: 3.871

OBJECTIVE: To describe the challenge of determining the correct diagnosis in a healthy adult male patient with a recent femoral fracture and a history of multiple bone fractures. METHODS: We present clinical, radiologic, laboratory, and histopathologic details in a patient with a history of recurrent fractures associated with minimal trauma. Moreover, the various types of osteopetrosis are reviewed. RESULTS: A 34-year-old African American man was in his usual state of good health when he fell hard on concrete. Immediately after the fall, he was able to bear weight, although pain prompted him ... Read More »
» Published in Endocr Pract. 2006 Sep-Oct;12(5):552-8.

11. Genetic testing of embryos: practices and perspectives of U.S. IVF clinics.
Match Strength: 3.715

OBJECTIVE: To better understand the current practices of IVF clinics regarding preimplantation genetic diagnosis (PGD) and explore the attitudes and opinions of clinic directors towards PGD. DESIGN: On-line Survey of 415 assisted reproductive technology (ART) clinics in the United States. The Survey had a valid response rate of 45 percent, or 190 clinics. SETTING: N/A PATIENTS: Respondents were medical directors, laboratory directors, IVF directors, or directors' designees of ART clinics offering IVF. INTERVENTIONS: N/A MAIN OUTCOME MEASURES: Practices and beliefs of IVF clinic directors with ... Read More »
» Published in Fertil Steril. 2006 Sep 19;

12. Rapid detection, serotyping and quantitation of dengue viruses by TaqMan real-time one-step RT-PCR.
Match Strength: 3.667

The use of the polymerase chain reaction (PCR) in molecular diagnosis is now accepted worldwide and has become an essential tool in the research laboratory. In the laboratory, a rapid detection, serotyping and quantitation, one-step real-time RT-PCR assay was developed for dengue virus using TaqMan probes. In this assay, a set of forward and reverse primers were designed targeting the serotype conserved region at the NS5 gene, at the same time flanking a variable region for all four serotypes which were used to design the serotype-specific TaqMan probes. This multiplex one-step RT-PCR assay ... Read More »
» Published in J Virol Methods. 2006 Dec;138(1-2):123-30. Epub 2006 Sep 26.

13. Specific primers for rapid detection of Microsporum audouinii by PCR in clinical samples.
Match Strength: 3.549

This report describes application of PCR fingerprinting to identify common species of dermatophytes using the microsatellite primers M13, (GACA)4, and (GTG)5. The initial PCR analysis rendered a specific DNA fragment for Microsporum audouinii, which was cloned and sequenced. Based on the sequencing data of this fragment, forward (MA_1F) and reverse (MA_1R) primers were designed and verified by PCR to establish their reliability in the diagnosis of M. audouinii. These primers produced a singular PCR band of 431 bp specific only to strains and isolates of M. audouinii, based on a global test of ... Read More »
» Published in J Clin Microbiol. 2006 Dec;44(12):4336-41. Epub 2006 Sep 27.

14. A path or a new road in laboratory diagnostics? Biological mass spectrometry: facts and perspectives.
Match Strength: 3.505

Proteins in tissues and biofluids and their many attributes define the proteome. Proteome can be directly correlated to known diseases and histological regions allowing the diagnosis and monitoring of disease progression as well predicting the patient's response to specific treatments. Proteomics performs large-scale, high-throughput characterization of the human proteome, among others by biological mass spectrometry. Proteinchip technology coupled with bioinformatics is able to screen any protein source for putative disease biomarkers from a small sample volume (microliter range) by surface ... Read More »
» Published in Pathol Oncol Res. 2006;12(3):178-83. Epub 2006 Sep 23.

15. Comparison of plain potassium hydroxide mounts, fungal cultures and nail plate biopsies in the diagnosis of onychomycosis.
Match Strength: 3.461

Objective: To compare the relative sensitivity of direct microscopy, fungal culture and nail plate biopsy in the diagnosis of onychomycosis. Design: Cross-sectional study. Place and Duration of Study: The Skin Department, Military Hospital, Rawalpindi from February 1998 to February 1999. Patients and Methods: A total of 50 patients who were suffering from different clinical variants of onychomycosis, irrespective of their age, gender, with or without simultaneous presence of systemic diseases, were subjected to laboratory investigations including direct microscopy with 20% potassium hydroxide ... Read More »
» Published in J Coll Physicians Surg Pak. 2006 Oct;16(10):641-4.

16. Superficial soft-tissue masses of the extremities.
Match Strength: 3.372

Superficial soft-tissue masses are among the most common indications for imaging of the extremities. A broad array of benign and malignant processes may be manifested in palpable cutaneous or subcutaneous masses or nodules. Most such lesions are treated with surgical excision, but some may be conservatively managed. The lesions can be differentiated according to their location in one or more skin layers (epidermis, dermis, and subcutis), their histologic composition, and the associated anatomic abnormality or disease process. Because the imaging characteristics of many benign soft-tissue ... Read More »
» Published in Radiographics. 2006 Sep-Oct;26(5):1289-304.

17. Evaluation of a glycerol-preserved antigen in the direct agglutination test for diagnosis of visceral leishmaniasis at rural level in eastern Sudan.
Match Strength: 3.286

Three-hundred and eight patients with suspected visceral leishmaniasis (VL) were received at Doka Hospital (eastern Sudan) during the period September 2004 to October 2005. The sensitivity and specificity of a glycerol-preserved (GP) antigen for VL diagnosis was assessed against the results of repeated lymph node aspiration and readings from a direct agglutination test (DAT) employing standard formaldehyde-fixed (FF) or freeze-dried (FD) antigen. Despite 13 months of storage at ambient temperature (28-47 degrees C), the GP antigen mean titres obtained from these 308 patients were no different ... Read More »
» Published in J Med Microbiol. 2006 Oct;55(Pt 10):1343-7.

18. Hospitalisation costs of cystic fibrosis.
Match Strength: 3.267

OBJECTIVE: To calculate per-case hospital costs for patients with cystic fibrosis under routine conditions from a healthcare provider's perspective; identify the impact of different cost categories; investigate whether cases with cystic fibrosis can be grouped into homogenous cost groups according to defined severity levels; and determine the value of specific factors as predictors of hospital cost variations. METHODS: All data were collected from cases (n = 131) admitted to an inpatient cystic fibrosis unit under routine conditions during a period of 6 months in 2004. All costs were ... Read More »
» Published in Pharmacoeconomics. 2006;24(10):999-1009.

19. Fatal agranulocytosis: the use of olanzapine in a patient with schizophrenia and myelodysplasia.
Match Strength: 3.144

Neutropenia and agranulocytosis are risks known to occur with phenothiazines and clozapine. The mechanisms responsible for these conditions currently remain unclear. To our knowledge, no case of fatal agranulocytosis as a result of olanzapine treatment was reported in the literature. Thus any case of severe neutropenia occurring in a patient receiving olanzapine is alarming to clinicians. First, a review of the literature produced 41 anecdotic cases of neutropenia or agranulocytosis during treatment with olanzapine (Zyprexa) reported in a total of 24 publications. Second, we report a case of ... Read More »
» Published in Prog Neuropsychopharmacol Biol Psychiatry. 2007 Jan 30;31(1):297-300. Epub 2006 Sep 15.

20. Current concepts on the pathogenesis of the antiphospholipid syndrome.
Match Strength: 3.122

The antiphospholipid syndrome (APS) is an important cause of acquired thrombophilia. It is characterized by the core clinical manifestations of thrombosis, either venous or arterial, and in women it can also be associated with recurrent fetal loss. The detection of persistently elevated levels of antiphospholipid antibodies (aPL Abs) is a requisite laboratory feature for the diagnosis to be made. The dominant antigenic targets in APS are beta 2-glycoprotein I (beta2-GPI) and prothrombin. There is an accumulating body of experimental evidence that suggests that specific subgroups of aPL Abs may ... Read More »
» Published in Blood. 2007 Jan 15;109(2):422-30. Epub 2006 Sep 19.

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